Comparative analysis of mouse-human hybrids with rearranged chromosomes 1 by in situ hybridization and southern blotting: high-resolution mapping of NRAS, NGFB, and AMY on human chromosome 1

The human protooncogene NRAS and the genes for the beta-subunit of nerve growth factor (NGFB) and for amylase (AMY) have previously been assigned to the proximal short arm of chromosome 1, but their precise positions have not been unequivocally established. By in situ hybridization of DNA probes for...

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Bibliographic Details
Published in:Somatic cell and molecular genetics 1984-11, Vol.10 (6), p.589-599
Main Authors: MUNKE, M, LINDGREN, V, DE MARTINVILLE, B, FRANCKE, U
Format: Article
Language:English
Subjects:
Amylases - genetics
Biological and medical sciences
Chromosome Mapping
Chromosomes, Human, 1-3
Cytogenetics
Fundamental and applied biological sciences. Psychology
Genes
Genetic Linkage
Genetics of eukaryotes. Biological and molecular evolution
Human
Humans
Hybrid Cells
Nerve Growth Factors - genetics
Nucleic Acid Hybridization
Oncogenes
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Summary:The human protooncogene NRAS and the genes for the beta-subunit of nerve growth factor (NGFB) and for amylase (AMY) have previously been assigned to the proximal short arm of chromosome 1, but their precise positions have not been unequivocally established. By in situ hybridization of DNA probes for the three genes, we have ascertained the location of complementary sequences in mouse-human somatic cell hybrids that contained translocations of chromosome 1. The results agreed with the presence or absence of the human sequences as determined by Southern blotting of hybrid cell DNA. The in situ data confirmed that the genes were present on the cytologically recognized rearranged chromosome. Compared to the autoradiographic silver grain distribution on normal human chromosome 1, our in situ results obtained with the translocation chromosomes allowed much greater precision of mapping. Both NRAS and NGFB map to band 1p22, and AMY was confirmed in band 1p21.
ISSN:0740-7750
1572-9931
DOI:10.1007/BF01535224