Characteristics of ATPase activity and the subunit composition of myosin in the conduction system of bovine heart

The ATPase activity, light chains and isoenzymes of myosin from specialized myocardial tissue (the A-V node, bundle of His, and right and left bundle branches) of bovine heart were compared with those of atrial and ventricular myosins. The order of Ca 2+-activated ATPase activity was atrial ⪢ specia...

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Bibliographic Details
Published in:FEBS letters 1984-12, Vol.178 (2), p.209-212
Main Authors: Tamura, Yoshiyuki, Nishimoto, Tetsuo, Miyakami, Sciji, Sako, Harunobu, Saito, Ken, Mori, Hiroyoshi
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - metabolism
adenosinetriphosphatase
Analytical, structural and metabolic biochemistry
Animals
ATPase
Biological and medical sciences
Calcium-Transporting ATPases - metabolism
Cardiac conduction system
Cattle
Contractile proteins
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
heart
Heart Atria - metabolism
Heart Ventricles - metabolism
Holoproteins
Isoenzymes - metabolism
Myocardium - metabolism
Myosin
Myosin isoenzyme
Myosin light chain
Myosins - metabolism
Proteins
Purkinje Fibers - metabolism
Rats
Specialized myocardial tissue
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Summary:The ATPase activity, light chains and isoenzymes of myosin from specialized myocardial tissue (the A-V node, bundle of His, and right and left bundle branches) of bovine heart were compared with those of atrial and ventricular myosins. The order of Ca 2+-activated ATPase activity was atrial ⪢ specialized myocardial tissue ⪢ ventricular myosin. SDS-polyacrylamide gel electrophoresis showed that myosin from the specialized myocardial tissue contained the light chains of both atrial and ventricular myosins. On the other hand, the specialized myocardial tissue contained one V 3 isomyosin and showed no difference from ventricular myocardial tissue on pyrophosphate gel.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80602-X