An EF-hands protein, centrin-1, is an EGTA-sensitive SUMO-interacting protein in mouse testis

A multifunctional calcium‐binding protein, centrin‐1, is specifically expressed in male germ cells, certain neurons and ciliated cells. We identified centrin‐1 as a protein interacting with SUMO‐2/3 using yeast two‐hybrid screening of a mouse testicular cDNA library. In bead halo assays, the interac...

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Bibliographic Details
Published in:Cell biochemistry and function 2010-10, Vol.28 (7), p.604-612
Main Authors: Tanaka, Niina, Goto, Miki, Kawasaki, Azusa, Sasano, Takashi, Eto, Ko, Nishi, Ryotaro, Sugasawa, Kaoru, Abe, Shinichi, Saitoh, Hisato
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
calcium signaling
Calcium-Binding Proteins - chemistry
Calcium-Binding Proteins - genetics
Calcium-Binding Proteins - metabolism
centrin-1
Centrosome - metabolism
Chelating Agents - chemistry
Chromosomal Proteins, Non-Histone - chemistry
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
EF Hand Motifs
Egtazic Acid - chemistry
Gene Library
HeLa Cells
Humans
Male
Mice
Mice, Inbred C57BL
Protein Transport
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - metabolism
Seminiferous Tubules - cytology
Seminiferous Tubules - metabolism
Small Ubiquitin-Related Modifier Proteins - genetics
Small Ubiquitin-Related Modifier Proteins - metabolism
spermatogenesis
Spermatogonia - cytology
Spermatogonia - metabolism
SUMO-2/3
Ubiquitins - genetics
Ubiquitins - metabolism
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Summary:A multifunctional calcium‐binding protein, centrin‐1, is specifically expressed in male germ cells, certain neurons and ciliated cells. We identified centrin‐1 as a protein interacting with SUMO‐2/3 using yeast two‐hybrid screening of a mouse testicular cDNA library. In bead halo assays, the interaction between centrin‐1 and SUMO‐2/3 was reduced in the presence of EGTA and facilitated by the addition of CaCl2. immunostaining of seminiferous tubules in 35‐day‐old mouse testes revealed that cells in the layer containing spermatogonia showed colocalization of SUMO‐2/3 with centrin‐1 in cytoplasmic spots. Identification of centrin‐1 as the EGTA‐sensitive SUMO‐2/3‐interacting protein indicates the possible role of calcium in modulating the centrin‐1–SUMO‐2/3 interaction and suggests the importance of this interaction in mouse testis. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.1698