Molecular typing of Salmonella enterica serovar Sofia in Australia by pulsed-field gel electrophoresis and repetitive element PCR typing

In this study, we used two molecular fingerprinting methods to investigate the genetic and clonal relationship shared by Australian Salmonella Sofia isolates. A total of 84 Australian Salm. Sofia isolates from various states in Australia were typed using pulsed-field gel electrophoresis (PFGE) (XbaI...

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Bibliographic Details
Published in:Journal of applied microbiology 2010-07, Vol.109 (1), p.292-303
Main Authors: Gan, E, Smooker, P.M, Coloe, P.J
Format: Article
Language:English
Subjects:
Australia
Biodegradation
Biological and medical sciences
Chemical fingerprinting
chicken
chickens
Deoxyribonuclease
Deoxyribonucleic acid
DNA
DNA fingerprinting
DNA Fingerprinting - methods
DNA Primers
DNA, Bacterial - analysis
DNase activity
Electrophoresis
Electrophoresis, Gel, Pulsed-Field - methods
Fundamental and applied biological sciences. Psychology
Gel electrophoresis
Genetic diversity
Lysis
Microbiology
Molecular Typing - methods
PFGE
Plugs
Polymerase chain reaction
Polymerase Chain Reaction - methods
rep-PCR
Salmonella
Salmonella enterica
Salmonella enterica - classification
Salmonella enterica - genetics
Salmonella enterica - isolation & purification
Salmonella enterica subsp. enterica serovar Sofia
Salmonella Sofia
Typing
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Summary:In this study, we used two molecular fingerprinting methods to investigate the genetic and clonal relationship shared by Australian Salmonella Sofia isolates. A total of 84 Australian Salm. Sofia isolates from various states in Australia were typed using pulsed-field gel electrophoresis (PFGE) (XbaI and SpeI) and repetitive element PCR (REP1R-I primer). The previous problem of DNA degradation of Salm. Sofia strains was solved by modifying the lysis solution used to treat the bacterial plugs, allowing Salm. Sofia to be subtyped using PFGE. Molecular typing of isolates resulted in the generation of eight XbaI, six SpeI and five REP1 pattern profiles. Individual typing methods showed low discrimination index values (
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2009.04655.x