Ultracytochemical localization of particulate guanylate cyclase in rat adrenal gland exposed to stimulation by porcine brain natriuretic peptide

We studied the cytochemical localization of particulate guanylate cyclase (GC) in rat adrenal gland after stimulation with porcine brain natriuretic peptide (pBNP) by electron microscopy. In the adrenal cortex, GC activity, as demonstrated by the presence of reaction product, was prevalently localiz...

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Bibliographic Details
Published in:The Histochemical journal 1993-05, Vol.25 (5), p.384-391
Main Authors: RAMBOTTI, M. G, ERCOLANI, P, SPRECA, A
Format: Article
Language:English
Subjects:
Adrenal Cortex - drug effects
Adrenal Cortex - enzymology
Adrenal Cortex - ultrastructure
Adrenal Medulla - drug effects
Adrenal Medulla - enzymology
Adrenal Medulla - ultrastructure
Adrenals. Interrenals
Animals
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Guanylate Cyclase - metabolism
Histocytochemistry
Microscopy, Electron
Morphology. Functional localizations
Natriuretic Peptide, Brain
Nerve Tissue Proteins - pharmacology
Rats
Vertebrates: endocrinology
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Summary:We studied the cytochemical localization of particulate guanylate cyclase (GC) in rat adrenal gland after stimulation with porcine brain natriuretic peptide (pBNP) by electron microscopy. In the adrenal cortex, GC activity, as demonstrated by the presence of reaction product, was prevalently localized to the zona glomerulosa and zona fasciculata, while the zona reticularis showed little GC reaction product. In the adrenal medulla, GC reaction product was present only in adrenalin-containing cells. All GC positivity was associated with intracellular membranes. No GC reaction product was detected in specimens incubated in media devoid of pBNP. In parallel samples incubated in the presence of rat atrial natriuretic factor (rANF), the distribution of rANF-stimulated GC activity was similar to that of pBNP-stimulated GC activity.
ISSN:0018-2214
1573-6865
DOI:10.1007/BF00159503