An operator binding‐negative mutation of Xyl repressor from Bacillus subtilis is trans dominant in Bacillus megaterium

We have selected a Bacillus subtilis 168‐borne xylR Ser to Leu mutation at position 41 of the encoded amino acid sequence showing a constitutive expression phenotype for the xyl operon. When cloned on a multi‐copy plasmid in a B. megaterium strain harbouring a single‐copy xylA‐lacZ fusion it leads t...

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Bibliographic Details
Published in:FEMS microbiology letters 1993-05, Vol.109 (1), p.81-84
Main Authors: Kauder, Christine, Allmansberger, Rudolf, Gärtner, Dagmar, Schmiedel, Dagmar, Hillen, Wolfgang
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillus megaterium
Bacillus megaterium - genetics
Bacillus subtilis
Bacillus subtilis - genetics
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Gene Expression
Genes, Dominant
Genetics
Microbiology
Molecular Sequence Data
Mutagenesis
Mutation
Operator Regions, Genetic
Plasmids - genetics
Repressor Proteins - genetics
Sequence Homology, Amino Acid
Xyl repressor
Xylose utilization
α‐Helix‐turn‐α‐helix motif
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Summary:We have selected a Bacillus subtilis 168‐borne xylR Ser to Leu mutation at position 41 of the encoded amino acid sequence showing a constitutive expression phenotype for the xyl operon. When cloned on a multi‐copy plasmid in a B. megaterium strain harbouring a single‐copy xylA‐lacZ fusion it leads to derepressor of β‐galactosidase expression. Thus, it is trans dominant over the endogenous xylR, indicating that Xyl repressor functions as a multimer. This result also supports the assumption that the mutation is in a putative α‐helix‐turn‐α‐helix operator binding motif of Xyl repressor.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1993.tb06147.x