The Growth of Concanavalin-A Activated, Lyt Selected Subsets in IL-2 Containing Supernatants

The growth properties of Con A activated, Lyt selected splenic T lymphocytes were examined by limiting dilution analysis and clonally by single cell picking. Under the conditions used, a comparable frequency of Lyt 1 + and Lyt 2 + cells grew after Con A activation in the presence of Con A rat spleen...

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Bibliographic Details
Published in:Immunobiology (1979) 1984-10, Vol.167 (4), p.365-375
Main Authors: Kuppers, R.C., Simon, M.M., Eichmann, K.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Antigens, Ly
Biological and medical sciences
Cell Division
Cell interactions
Concanavalin A - pharmacology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Interleukin-2 - immunology
Kinetics
Lymphocyte Activation
Mice
Mice, Inbred C57BL
T-Lymphocytes - classification
T-Lymphocytes - immunology
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Summary:The growth properties of Con A activated, Lyt selected splenic T lymphocytes were examined by limiting dilution analysis and clonally by single cell picking. Under the conditions used, a comparable frequency of Lyt 1 + and Lyt 2 + cells grew after Con A activation in the presence of Con A rat spleen supernatant. At a clonal level, however, the growth of these subsets differed qualitatively and quantitatively. While Lyt 2 + cells obtained clone sizes of several hundred cells, Lyt 1 + clone sizes were usually < 100 cells, and many clones aborted their growth after a few days. Morphologically, the Lyt 1 + cell was smaller and usually showed fewer pseudopodial protusions as compared to the Lyt 2 + cell.
ISSN:0171-2985
1878-3279
DOI:10.1016/S0171-2985(84)80008-X