Effect of Amiloride on Sodium-Calcium Exchange Activity in Rat Cardiac Myocytes

The effect of amiloride on Na +/Ca 2+ exchange activity and adenylate energy charge in isolated rat cardiac myocytes has been studied. Amiloride inhibits Na +/Ca 2+ exchange activity in rat cardiac myocytes with an IC 50 of 1.76 mM when added to the external side of the cell membrane, whereas it is...

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Bibliographic Details
Published in:Pharmacological research 1993-04, Vol.27 (3), p.227-232
Main Authors: Antolini, M., Trevisi, L., Debetto, P., Luciani, S.
Format: Article
Language:English
Subjects:
Adenosine Monophosphate - metabolism
Amiloride - pharmacokinetics
Amiloride - pharmacology
amiloride, Na +/Ca 2+ exchange, adenylate energy charge
Animals
Biological and medical sciences
Carrier Proteins - antagonists & inhibitors
Carrier Proteins - drug effects
Carrier Proteins - metabolism
Energy Metabolism
Heart - drug effects
Intracellular Fluid - metabolism
Kinetics
Male
Medical sciences
Myocardium - cytology
Myocardium - metabolism
Pharmacology. Drug treatments
Rats
Rats, Wistar
Sarcolemma - drug effects
Sarcolemma - metabolism
Sodium-Calcium Exchanger
Urinary system
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Summary:The effect of amiloride on Na +/Ca 2+ exchange activity and adenylate energy charge in isolated rat cardiac myocytes has been studied. Amiloride inhibits Na +/Ca 2+ exchange activity in rat cardiac myocytes with an IC 50 of 1.76 mM when added to the external side of the cell membrane, whereas it is a weaker inhibitor when present at the cytoplasmic side. The adenylate energy charge in rat cardiac myocytes is unaffected by amiloride.
ISSN:1043-6618
1096-1186
DOI:10.1006/phrs.1993.1021