Molecular cloning, expression, and gene localization of a fourth melanocortin receptor

The recent cloning of three melanocortin receptors suggests an unexpected diversity in this family of seven transmembrane G-protein linked receptors. Herein, we report the cloning, expression, and gene localization of a fourth human melanocortin receptor, the melanocortin-4 receptor. By Northern blo...

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Bibliographic Details
Published in:The Journal of biological chemistry 1993-07, Vol.268 (20), p.15174-15179
Main Authors: GANTZ, I, MIWA, H, KONDA, Y, SHIMOTO, Y, TASHIRO, T, WATSON, S. J, DELVALLE, J, YAMADA, T
Format: Article
Language:English
Subjects:
Adrenocorticotropic Hormone - pharmacology
alpha-MSH - pharmacology
Amino Acid Sequence
Animals
Biological and medical sciences
brain
Cell receptors
Cell structures and functions
Cells, Cultured
Cloning, Molecular
Cyclic AMP - metabolism
DNA, Single-Stranded
Fundamental and applied biological sciences. Psychology
genes
Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors
Humans
Male
man
melanocortin receptors
Mice
Molecular and cellular biology
Molecular Sequence Data
nucleotide sequence
predictions
Receptor, Melanocortin, Type 4
Receptors, Cell Surface - chemistry
Receptors, Cell Surface - drug effects
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Summary:The recent cloning of three melanocortin receptors suggests an unexpected diversity in this family of seven transmembrane G-protein linked receptors. Herein, we report the cloning, expression, and gene localization of a fourth human melanocortin receptor, the melanocortin-4 receptor. By Northern blot analysis and in situ hybridization, this receptor is expressed primarily in the brain, but its expression is notably absent in the adrenal cortex, melanocytes, and placenta. Agonist stimulation of COS-1 cells transiently transfected and L-cells permanently transfected with the coding region of the cloned melanocortin-4 receptor leads to increases in intracellular cyclic 3',5'-adenosine monophosphate. The profile of the responses of the melanocortin-4 receptor to different melanocortins distinguishes it from melanocortin receptors previously described. Using the technique of fluorescent in situ hybridization, the gene encoding the melanocortin-4 receptor was localized to chromosome 18 (q21.3).
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(18)82452-8