Annexin 1 is present in different molecular forms in rat cerebral cortex

Previously we have purified annexin 1 [J. Neurochem. 56 (1991) 1985-1986] from pig cerebral cortex as a monomeric protein of 37 kDa. Here, the localization of annexin 1 was investigated in subcellular fractionations of rat cerebral cortex using immunodetection by a specific antibody. In contrast to...

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Bibliographic Details
Published in:FEBS letters 1993-07, Vol.327 (1), p.41-44
Main Authors: Pradel, L.A., Rendon, A.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Annexin 1
Annexin A1 - analysis
Annexin A1 - isolation & purification
Binding and carrier proteins
Biological and medical sciences
Calcium-binding protein
Calpactin 2
Cerebral Cortex - chemistry
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Lipocortin 1
Molecular Weight
Phospholipid-binding protein
Proteins
Rats
Subcellular Fractions
Synaptic Membranes - chemistry
Synaptic plasma membrane
Synaptophysin - analysis
Transglutamination
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Summary:Previously we have purified annexin 1 [J. Neurochem. 56 (1991) 1985-1986] from pig cerebral cortex as a monomeric protein of 37 kDa. Here, the localization of annexin 1 was investigated in subcellular fractionations of rat cerebral cortex using immunodetection by a specific antibody. In contrast to synaptophysin, a specific synaptic vesicle integral membrane protein, annexin 1 is located in the synaptic plasma membrane fraction where it appears on SDS-PAGE as a polypeptide of 74 kDa. Annexin 1 is extracted also as a 74 kDa polypeptide from the purified synaptic plasma membranes. These results suggest for the 74 kDa molecular form an enzymatic dimerization of annexin 1 when associated to the membrane.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)81035-X