Isolation and mapping of discrete DXS101 loci in Xq22 near the X-linked agammaglobulinaemia gene locus

The X-linked agammaglobulinaemia (XLA) gene locus has previously been mapped to Xq22 in genetic linkage studies. The DXS101 locus has shown no recombinations with XLA in the ten informative meioses investigated so far. The DXS101 sequence, recognised by the cX52.5 plasmid, is moderately repeated in...

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Bibliographic Details
Published in:Human genetics 1993-07, Vol.91 (6), p.605-608
Main Authors: O'REILLY, M.-A. J, SWEATMAN, A. K, BRADLEY, L. D, ALTERMAN, L. A, LOVERING, R, MALCOLM, S, LEVINSKY, R. J, KINNON, C
Format: Article
Language:English
Subjects:
Agammaglobulinemia - genetics
Base Sequence
Biological and medical sciences
Chromosome Mapping
Cosmids
Genetic Linkage
Humans
Immunodeficiencies. Immunoglobulinopathies
Immunoglobulinopathies
Immunopathology
Medical sciences
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
X Chromosome
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Summary:The X-linked agammaglobulinaemia (XLA) gene locus has previously been mapped to Xq22 in genetic linkage studies. The DXS101 locus has shown no recombinations with XLA in the ten informative meioses investigated so far. The DXS101 sequence, recognised by the cX52.5 plasmid, is moderately repeated in Xq22. We have isolated cosmids which contain this sequence; two copies of which have been found to lie near DXS178 and XLA, and a third copy which lies near the PLP gene, distal to these loci. We have used the cosmids to generate probes which should be of use for RFLP analysis, and thus in both prenatal diagnosis and carrier testing for XLA, and in constructing a genetic map of this region. These probes will also be used to complement the genetic map in the construction of a complete physical map of Xq22.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF00205088