HPLC Determination of d-Glucaric Acid in Human Urine

An isocratic HPLC method has been developed for the direct measurement of d-glucaric acid in human urine. Pretreatment of urine with a boronic acid gel removed many interfering substances, including l-ascorbic acid and d-glucuronic acid. This method has a detection limit of 10µM d-glucaric acid (app...

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Bibliographic Details
Published in:Journal of analytical toxicology 1993-05, Vol.17 (3), p.146-150
Main Authors: Poon, Raymond, Villeneuve, David C., Chu, Ih, Kinach, Robert
Format: Article
Language:English
Subjects:
Adult
Analysis
Ascorbic acid
Ascorbic Acid - urine
Biological and medical sciences
Chromatography, High Pressure Liquid
Colorimetry
Creatinine
Creatinine - blood
Female
General pharmacology
Glucaric Acid - urine
Glucuronates - urine
Glucuronic Acid
High-performance liquid chromatography
Humans
Indicators and Reagents
Male
Medical sciences
Middle Aged
Pharmacology. Drug treatments
Sex Characteristics
Urine
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Summary:An isocratic HPLC method has been developed for the direct measurement of d-glucaric acid in human urine. Pretreatment of urine with a boronic acid gel removed many interfering substances, including l-ascorbic acid and d-glucuronic acid. This method has a detection limit of 10µM d-glucaric acid (approximately 7 µmoles/g creatinine). The run-to-run precisions were 9.1% and 7.7% at urinary d-glucaric acid concentrations of 41 and 219 µmoles/g creatinine, respectively. Urinary d-glucaric acid concentrations in normal adults were found to cover a range of 15 to 89 µmoles/g creatinine (mean = 47 µmoles/g creatinine). The sensitivity of this method in detecting abnormal elevations in d-glucaric acid was demonstrated through its ability to measure changes in urinary concentrations with time after ingestion of d-glucuronolactone.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/17.3.146