Immunogenicity of a hybrid Plasmodium falciparum, malaria antigen

A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC...

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Bibliographic Details
Published in:Parasitology 1993-06, Vol.106 (5), p.451-457
Main Authors: Lockyer, M. J., Cooper, H., Tite, J., Rowan, W., Crowe, J. S.
Format: Article
Language:English
Subjects:
adjuvants
Adjuvants, Immunologic
Amino Acid Sequence
Animals
Antibodies, Protozoan - biosynthesis
antigens
Antigens, Protozoan - immunology
Antigens, Surface - immunology
baculovirus
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Cell Line
circumsporozoite protein
Fundamental and applied biological sciences. Psychology
hybrid antigen
Immunization
Immunization, Secondary
Invertebrates
Iscoms
ISCOMs - immunology
Lymphocyte Activation
malaria
Merozoite Surface Protein 1
Mice
Mice, Inbred C57BL
Mice, Inbred CBA
Mice, Inbred Strains
Molecular Sequence Data
Plasmodium falciparum - immunology
Protein Precursors - immunology
Protozoa
Protozoan Proteins - immunology
Protozoan Vaccines - immunology
Rabbits
Recombinant Fusion Proteins - immunology
T-Lymphocytes - immunology
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Summary:A recombinant baculovirus-expressed hybrid protein containing epitopes for the C-terminal fragment of the Plasmodium falciparum precursor to the major merozoite surface antigens (PMMSA) and the tetrapeptide repeats of the circumsporozoite protein (CSP) was assessed for its immunogenicity. Murine MHC-II restriction of the antibody response to the CSP repeats was not overcome by the PMMSA component, the response to which showed no restriction. In an adjuvant trial the highest antibody titres in rabbits to both components of the hybrid were obtained using Freund's adjuvant. Lack of a boosting antibody response to the CSP repeats appeared to be linked to the conformation of the PMMSA component. Formulation of the hybrid protein into Iscoms gave antibody titres of only short duration to both components.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000076733