FABMS/derivatisation strategies for the analysis of heparin-derived oligosaccharides

Derivatisation/FABMS strategies applicable to the structure analysis of low microgramme quantities of heparin-derived oligosaccharides are described. Negative and positive FAB data from permethyl derivatives and positive FAB data from the products of subsequent methanolysis are reported for sulfated...

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Bibliographic Details
Published in:Carbohydrate research 1993-06, Vol.244 (2), p.205-223
Main Authors: Khoo, Kay-Hooi, Morris, Howard R., McDowell, Roy A., Dell, Anne, Maccarana, Marco, Lindahl, Ulf
Format: Article
Language:English
Subjects:
Acetylation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrate Sequence
carbohydrate structure
Carbohydrates
Deamination
fast atom bombardment mass spectrometry
Fundamental and applied biological sciences. Psychology
heparin
Heparin - chemistry
Holosides
mass spectrometry
Methanol - chemistry
Methylation
Molecular Sequence Data
Molecular Structure
oligosaccharides
Oligosaccharides - analysis
Other biological molecules
proteoglycans
spectral analysis
Spectrometry, Mass, Fast Atom Bombardment
structure
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Summary:Derivatisation/FABMS strategies applicable to the structure analysis of low microgramme quantities of heparin-derived oligosaccharides are described. Negative and positive FAB data from permethyl derivatives and positive FAB data from the products of subsequent methanolysis are reported for sulfated tetrasaccharides prepared by nitropus acid degradation of heparin. The preparation and FAB behaviour of acetylated derivatives of sulfated oligosaccharides are described for the first time, and the stability of the sulfate groups to base-catalysed acetylation is demonstrated. The acetylation/FABMS methodology, which yields high quality data, shows promise for the characterisation of a wide range of sulfated glycoconjugates.
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(83)85002-2