Fast atom bombardment mass spectrometry of glycosphingolipids. Glycosphingolipids containing neutral sugars

Natural and synthetic glycosphingolipids containing neutral sugars have been analyzed by positive and negative ion fast atom bombardment mass spectrometry. Basic structural characterization including saccharide size and sequence and ceramide composition is possible on the basis of the fragment ions...

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Bibliographic Details
Published in:Biochemistry (Easton) 1984-11, Vol.23 (24), p.5706-5713
Main Authors: Hemling, Mark E, Yu, Robert K, Sedgwick, R. Donald, Rinehart, Kenneth L
Format: Article
Language:English
Subjects:
atomic absorption spectroscopy
Biological and medical sciences
Carbohydrates
Ceramides
Cerebrosides
Chromatography, Gas
erythrocytes
Erythrocytes - analysis
Fatty Acids - analysis
Fundamental and applied biological sciences. Psychology
Gaucher Disease - metabolism
glycosphingolipids
Glycosphingolipids - analysis
Humans
man
Mass Spectrometry
Molecular biophysics
Spectroscopy : techniques and spectras
Spleen - analysis
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Summary:Natural and synthetic glycosphingolipids containing neutral sugars have been analyzed by positive and negative ion fast atom bombardment mass spectrometry. Basic structural characterization including saccharide size and sequence and ceramide composition is possible on the basis of the fragment ions observed. The degree of fragmentation could be increased by using higher sample concentrations and lower fast atom beam energies. Commercially available synthetic compounds that had been presumed to be pure were shown to contain homologous fatty acids. Mixtures of glycosphingolipids such as those obtained from Gaucher's spleen and from human erythrocytes can be characterized and quantitated.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00319a008