Sharing of an HLA-B27-restricted H-Y antigen between rat and mouse

The purpose of this work was twofold: 1) to learn whether rats transgenic for HLA-B27 and the human beta 2-microglobulin gene HB2M can mount B27-restricted cytolytic T lymphocyte (CTL) responses to the male H-Y antigen, and 2) to learn whether such CTLs would recognize both rat and mouse H-Y in the...

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Bibliographic Details
Published in:Immunogenetics (New York) 1993-07, Vol.38 (5), p.351-358
Main Authors: SIMMONS, W. A, TAUROG, J. D, HAMMER, R. E, BREBAN, M
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Antibodies, Monoclonal - immunology
Antigens
beta 2-Microglobulin - genetics
Biological and medical sciences
CD8 Antigens - physiology
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
H-Y Antigen - immunology
Histocompatibility antigens (hla, h-2 and other systems)
HLA-B27 Antigen - genetics
HLA-B27 Antigen - physiology
Male
Mice
Molecular immunology
Rats
T-Lymphocytes, Cytotoxic - immunology
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Summary:The purpose of this work was twofold: 1) to learn whether rats transgenic for HLA-B27 and the human beta 2-microglobulin gene HB2M can mount B27-restricted cytolytic T lymphocyte (CTL) responses to the male H-Y antigen, and 2) to learn whether such CTLs would recognize both rat and mouse H-Y in the context of HLA-B27. Female rats of the B27/HB2M transgenic line 21-4L were primed in vivo with cells from males of the same line. CTL effectors were generated from lymph node cells of these females following culture with irradiated antigen-presenting cells from either male 21-4L rats or male mice of the B27/HB2M transgenic 56-3 line. The CTLs showed male-specific, B27-specific lysis of both rat and mouse targets. Lysis of B27 targets was inhibitable by monoclonal antibodies specific for B27 or rat CD8. Specific lysis of male B27 rat and mouse targets was inhibitable equally by either rat or mouse male B27 cold targets, but not significantly by female or nontransgenic cold targets. The B27-restricted CTLs neither recognized nor were inhibited by B27+ or B27- male or female human targets. These results demonstrate that CD8+, B27-restricted, anti-H-Y CTLs recognize an evolutionarily conserved H-Y peptide antigen in both rats and mice. In addition, they establish the transgenic rat as a model system for examining the T-cell response to antigen presented by class I HLA molecules.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00210477