Melastin, a Novel Product of Streptomyces That Selectively Inhibits Leukemia Cell Growth

In the course of our screening for new immunomodulators, a novel compound, melastin, was purified from the culture broth of Streptomyces. Melastin was purified through adsorption to Diaion HP-20, ethanol precipitation, and anion exchange column as a brown powder. The molecular weight was estimated a...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1993, Vol.57 (6), p.969-972
Main Authors: Magae, Junji, Tsuji, Ryohei F., Wang, Zhang, Kataoka, Takao, Lee, Mi-Heon, Hanada, Toshihiko, Kurisaki, Tomohiro, Uramoto, Masakazu, Yamasaki, Makari, Nagai, Kazuo
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Animals
Antineoplastic Agents - chemistry
Antineoplastic Agents - isolation & purification
Antineoplastic Agents - pharmacology
Bacterial Proteins - chemistry
Bacterial Proteins - isolation & purification
Bacterial Proteins - pharmacology
Biological and medical sciences
Biotechnology
Cell Division - drug effects
Cell Line, Transformed
Cells, Cultured
Chromatography, High Pressure Liquid
Drug Screening Assays, Antitumor
Immunologic Factors - chemistry
Immunologic Factors - isolation & purification
Immunologic Factors - pharmacology
Immunomodulators
Leukemia, Experimental - pathology
Leukocytes - cytology
Leukocytes - drug effects
Lymphocyte Activation - drug effects
Medical sciences
Mice
Pharmacology. Drug treatments
Streptomyces
Streptomyces - metabolism
Tumor Cells, Cultured
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Summary:In the course of our screening for new immunomodulators, a novel compound, melastin, was purified from the culture broth of Streptomyces. Melastin was purified through adsorption to Diaion HP-20, ethanol precipitation, and anion exchange column as a brown powder. The molecular weight was estimated as 5000 ± 3000 by gel filtration HPLC. Melastin suppressed lipopolysaccharide (LPS) induced blastogenesis of B cells more profoundly than concanavalin A (con A)- or phytohemagglutinin (PHA)-induced blastogenesis of T cells. Moreover, it selectively inhibited the growth of several leukemia cells as compared with interleukin-dependent nontransformed leukocytes. No selectivity was observed between nontransformed fibroblasts and their oncogene-transformed variants. Melastin did not selectively inhibit macromolecule synthesis of leukemia cells.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.57.969