Identification of IP-10 and IL-5 as Proteins Differentially Expressed in Human Complicated and Uncomplicated Carotid Atherosclerotic Plaques

Inflammation plays a crucial role in the development and progression of atherosclerotic plaques. The aim of this study is to compare culture supernatants from uncomplicated and complicated carotid atherosclerotic plaques by a multiplex approach, to assess the molecular mediators associated with a co...

Full description

Saved in:
Bibliographic Details
Published in:International journal of immunopathology and pharmacology 2010-07, Vol.23 (3), p.775-782
Main Authors: Profumo, E., Buttari, B., Tosti, M.E., Alessandri, C., Valesini, G., Marcuccio, L., Tesori, C., Capoano, R., Salvati, B., Riganò, R.
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biomarkers
Carotid Artery Diseases - metabolism
Carotid Artery Diseases - surgery
Chemokine CXCL10 - biosynthesis
Chemokines - biosynthesis
Cytokines - biosynthesis
Endarterectomy, Carotid
Female
Humans
Intercellular Signaling Peptides and Proteins - biosynthesis
Interleukin-5 - biosynthesis
Male
Middle Aged
Phenotype
Citations: Items that this one cites
Items that cite this one
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Inflammation plays a crucial role in the development and progression of atherosclerotic plaques. The aim of this study is to compare culture supernatants from uncomplicated and complicated carotid atherosclerotic plaques by a multiplex approach, to assess the molecular mediators associated with a complicated plaque phenotype. Atherosclerotic plaques were obtained from 17 patients undergoing carotid endarterectomy. Supernatants from plaque cultures were evaluated by Bio-Plex cytokine assay to determine 27 pro- and anti-inflammatory cytokines, chemokines and growth factors. Complicated plaques secreted higher levels of IP-10 (p = 0.027) and lower levels of IL-5 (p = 0.045) than did uncomplicated ones. Distinctive secretory patterns of cytokines, chemokines and growth factors were present in the two types of plaque. Our study identifies IP-10 and IL-5 as proteins differentiating complicated and uncomplicated plaques from human carotid arteries and provides new insights into the interplay of molecular mediators with atherosclerotic plaque progression.
ISSN:0394-6320
2058-7384
DOI:10.1177/039463201002300311