Profile of hepatocyte apoptosis and bile lakes before and after bile duct decompression in severe obstructive jaundice patients

BACKGROUND:Excessive hepatocyte apoptosis and bile lakes in severe obstructive jaundice might impair liver functions.Although decompression of the bile duct has been reported to improve liver functions in animal studies,the mechanism of obstruction differs from that in humans.This study aimed to det...

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Bibliographic Details
Published in:Hepatobiliary & pancreatic diseases international 2010-10, Vol.9 (5), p.520-523
Main Authors: Lalisang, Toar J M, Sjamsuhidajat, Raden, Siregar, Nurjati C, Taher, Akmal
Format: Article
Language:English
Subjects:
Adult
Aged
apoptosis
Apoptosis - physiology
Bile
Bile Ducts - surgery
Cholecystostomy
Cholestasis - complications
Cholestasis - pathology
Cholestasis - physiopathology
Chronic Disease
decompression
Decompression, Surgical
duct
Female
hepatocyte
Hepatocytes - pathology
Humans
jaundice
Jaundice, Obstructive - complications
Jaundice, Obstructive - pathology
Jaundice, Obstructive - physiopathology
Jaundice, Obstructive - surgery
lakes
Liver Diseases - etiology
Liver Diseases - pathology
Male
Middle Aged
Neoplasms - surgery
obstructive
TUNEL
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Summary:BACKGROUND:Excessive hepatocyte apoptosis and bile lakes in severe obstructive jaundice might impair liver functions.Although decompression of the bile duct has been reported to improve liver functions in animal studies,the mechanism of obstruction differs from that in humans.This study aimed to determine the profiles of hepatocyte apoptosis and bile lakes following bile duct decompression in patients with severe obstructive jaundice in the clinical setting.METHODS:We conducted a "before and after study" on severe obstructive jaundice patients as a model of inhibition of the excessive process by bile duct decompression.Specimens of liver biopsies were taken before and after decompression of the bile duct and then stained by terminal deoxynucleotide transferase-mediated dUTP nick end-labeling(TUNEL) to identify hepatocyte apoptosis and by hematoxilin-eosin(HE) to identify bile lakes.All measurements were independently done by 2 observers.RESULTS:Twenty-one severe obstructive jaundice patients were included.In all patients,excessive hepatocyte apoptosis and bile lakes were apparent.After decompression,the hepatocyte apoptosis index decreased from 53.1(SD 105) to 11.7(SD 13.6)(P<0.05),and the bile lakes from 23.6(SD 14.8) to 10.9(SD 6.9)(P<0.05).CONCLUSION:Bile duct decompression improves hepatocyte apoptosis and bile lakes in cases of severe obstructive jaundice,similar to the findings in animal studies.
ISSN:1499-3872