Biochemical comparison of brain glycosaminoglycans between normal and reeler mutant mice

Glycosaminoglycans (GAGs) were isolated from the brains of reeler and normal mice on postnatal days 13 and 20. The GAG content of the reeler mouse brain, based upon the amount of DNA, was about 150% that of the normal mouse brain on both days. The GAGs consisted of chondroitin sulfate (CS), heparan...

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Bibliographic Details
Published in:Neuroscience research 1993-05, Vol.16 (4), p.287-292
Main Authors: Matsui, Fumiko, Oohira, Atsuhiko, Shoji, Ryujiro, Kariya, Yutaka, Yoshida, Keiichi
Format: Article
Language:English
Subjects:
Animals
Biochemistry and metabolism
Biological and medical sciences
Brain
Brain - metabolism
Central nervous system
Chondroitin sulfate
Chondroitin Sulfate Proteoglycans - chemistry
Chondroitin Sulfates - chemistry
Chromatography, High Pressure Liquid
Disaccharides - chemistry
DNA - metabolism
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Glycosaminoglycan
Glycosaminoglycans - chemistry
Glycosaminoglycans - metabolism
Heparan sulfate
Heparitin Sulfate - chemistry
Mice
Mice, Neurologic Mutants - metabolism
Proteins - analysis
Proteoglycan
Reeler mouse
Reference Values
Solubility
Vertebrates: nervous system and sense organs
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Summary:Glycosaminoglycans (GAGs) were isolated from the brains of reeler and normal mice on postnatal days 13 and 20. The GAG content of the reeler mouse brain, based upon the amount of DNA, was about 150% that of the normal mouse brain on both days. The GAGs consisted of chondroitin sulfate (CS), heparan sulfate (HS), hyaluronic acid (HA) and polysialosyl glycopeptides. There was no significant difference in the composition of GAGs isolated from either reeler or normal brain. Repeating disaccharide compositions of CS and HS were also similar in reeler and normal brains. Core proteins of brain chondroitin sulfate proteoglycans (CSPGs), solubilized with phosphate buffered saline, were prepared by digesting purified CSPGs with chondroitinase ABC, and were analyzed by SDS-polyacrylamide slab gel electrophoresis. There was no difference in the composition of core proteins from either reeler or normal brain. These results indicate that, although the GAG content of the reeler mouse brain is higher than the normal, all structural parameters of GAGs/CSPGs so far examined were normal. The rate of synthesis and/or degradation of brain GAGs may be affected in the mutant mouse brain.
ISSN:0168-0102
1872-8111
DOI:10.1016/0168-0102(93)90039-S