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Characterization and mapping of simian varicella virus transcripts
Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Science, Little Rock, Arkansas 72205, U.S.A. The size and genomic location of viral transcripts expressed in simian varicella virus (SVV)-infected Vero cells were determined. Total cellular RNA an...
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Published in: | Journal of general virology 1993-08, Vol.74 (8), p.1639-1643 |
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container_title | Journal of general virology |
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creator | Gray, Wayne L Gusick, Nanette Fletcher, Thomas M Pumphrey, Carla Y |
description | Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Science, Little Rock, Arkansas 72205, U.S.A.
The size and genomic location of viral transcripts expressed in simian varicella virus (SVV)-infected Vero cells were determined. Total cellular RNA and polyadenylated RNA were isolated from SVV-infected and mock-infected Vero cells. Viral transcripts were detected by Northern blot hybridization analysis using overlapping SVV DNA probes representative of the entire SVV genome. The results indicated that all regions of the SVV genome are transcribed during SVV infection in vitro . At least 53 distinct viral RNA species ranging in size from 9·2 to 0·8 kb were detected. DNA probes derived from the SVV DNA long (L) and short (S) components hybridized to 44 RNAs (9·2 to 0·8 kb) and nine RNAs (4·9 to 0·8 kb), respectively. A transcript map of the SVV genome was constructed. The comparison made between the transcript maps of SVV and varicellazoster virus (VZV) provides further support that the SVV and VZV genomes have an analogous gene organization.
Received 3 February 1993;
accepted 10 March 1993. |
doi_str_mv | 10.1099/0022-1317-74-8-1639 |
format | article |
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The size and genomic location of viral transcripts expressed in simian varicella virus (SVV)-infected Vero cells were determined. Total cellular RNA and polyadenylated RNA were isolated from SVV-infected and mock-infected Vero cells. Viral transcripts were detected by Northern blot hybridization analysis using overlapping SVV DNA probes representative of the entire SVV genome. The results indicated that all regions of the SVV genome are transcribed during SVV infection in vitro . At least 53 distinct viral RNA species ranging in size from 9·2 to 0·8 kb were detected. DNA probes derived from the SVV DNA long (L) and short (S) components hybridized to 44 RNAs (9·2 to 0·8 kb) and nine RNAs (4·9 to 0·8 kb), respectively. A transcript map of the SVV genome was constructed. The comparison made between the transcript maps of SVV and varicellazoster virus (VZV) provides further support that the SVV and VZV genomes have an analogous gene organization.
Received 3 February 1993;
accepted 10 March 1993.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-74-8-1639</identifier><identifier>PMID: 8393915</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Biological and medical sciences ; Cercopithecus aethiops ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; Genetics ; Herpesvirus 3, Human - genetics ; Microbiology ; Restriction Mapping ; RNA, Messenger - genetics ; RNA, Viral - genetics ; Transcription, Genetic - genetics ; Vero Cells ; Virology</subject><ispartof>Journal of general virology, 1993-08, Vol.74 (8), p.1639-1643</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-8b017861b7567ca52255906d06db91cb4a44b5bff9f3e782cf9637727509a08a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3751724$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8393915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gray, Wayne L</creatorcontrib><creatorcontrib>Gusick, Nanette</creatorcontrib><creatorcontrib>Fletcher, Thomas M</creatorcontrib><creatorcontrib>Pumphrey, Carla Y</creatorcontrib><title>Characterization and mapping of simian varicella virus transcripts</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Science, Little Rock, Arkansas 72205, U.S.A.
The size and genomic location of viral transcripts expressed in simian varicella virus (SVV)-infected Vero cells were determined. Total cellular RNA and polyadenylated RNA were isolated from SVV-infected and mock-infected Vero cells. Viral transcripts were detected by Northern blot hybridization analysis using overlapping SVV DNA probes representative of the entire SVV genome. The results indicated that all regions of the SVV genome are transcribed during SVV infection in vitro . At least 53 distinct viral RNA species ranging in size from 9·2 to 0·8 kb were detected. DNA probes derived from the SVV DNA long (L) and short (S) components hybridized to 44 RNAs (9·2 to 0·8 kb) and nine RNAs (4·9 to 0·8 kb), respectively. A transcript map of the SVV genome was constructed. The comparison made between the transcript maps of SVV and varicellazoster virus (VZV) provides further support that the SVV and VZV genomes have an analogous gene organization.
Received 3 February 1993;
accepted 10 March 1993.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cercopithecus aethiops</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>Genetics</subject><subject>Herpesvirus 3, Human - genetics</subject><subject>Microbiology</subject><subject>Restriction Mapping</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Viral - genetics</subject><subject>Transcription, Genetic - genetics</subject><subject>Vero Cells</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNqFkMlKA0EQhhtRNC5PIMIcRLyM9r4cNbiB4EXPTU2nJ2nJLHZPIvr0dkyIR6GgDv9XCx9CpwRfEWzMNcaUloQRVSpe6pJIZnbQiHApSprzXTTaEgfoMKV3jAnnQu2jfc0MM0SM0O14BhHc4GP4hiF0bQHtpGig70M7Lbq6SKEJ0BZLiMH5-RyKZYiLVAwR2uRi6Id0jPZqmCd_sulH6O3-7nX8WD6_PDyNb55Lx5keSl1horQklRJSORCUCmGwnOSqDHEVB84rUdW1qZlXmrraSKYUVQIbwBrYEbpY7-1j97HwabBNSL8_tb5bJKuENoZQ9S9IpORYGp1BtgZd7FKKvrZ9DA3EL0uwXSm2K4F2JdAqbrVdKc5TZ5v1i6rxk-3MxmnOzzc5JAfzOptyIW0xpgRRlGfsco3NwnT2GaK3U982Ib9Shc5my38XfwCF7pDa</recordid><startdate>19930801</startdate><enddate>19930801</enddate><creator>Gray, Wayne L</creator><creator>Gusick, Nanette</creator><creator>Fletcher, Thomas M</creator><creator>Pumphrey, Carla Y</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19930801</creationdate><title>Characterization and mapping of simian varicella virus transcripts</title><author>Gray, Wayne L ; Gusick, Nanette ; Fletcher, Thomas M ; Pumphrey, Carla Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-8b017861b7567ca52255906d06db91cb4a44b5bff9f3e782cf9637727509a08a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cercopithecus aethiops</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - genetics</topic><topic>Genetics</topic><topic>Herpesvirus 3, Human - genetics</topic><topic>Microbiology</topic><topic>Restriction Mapping</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Viral - genetics</topic><topic>Transcription, Genetic - genetics</topic><topic>Vero Cells</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gray, Wayne L</creatorcontrib><creatorcontrib>Gusick, Nanette</creatorcontrib><creatorcontrib>Fletcher, Thomas M</creatorcontrib><creatorcontrib>Pumphrey, Carla Y</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gray, Wayne L</au><au>Gusick, Nanette</au><au>Fletcher, Thomas M</au><au>Pumphrey, Carla Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and mapping of simian varicella virus transcripts</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1993-08-01</date><risdate>1993</risdate><volume>74</volume><issue>8</issue><spage>1639</spage><epage>1643</epage><pages>1639-1643</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Department of Microbiology and Immunology, 4301 West Markham Street, University of Arkansas for Medical Science, Little Rock, Arkansas 72205, U.S.A.
The size and genomic location of viral transcripts expressed in simian varicella virus (SVV)-infected Vero cells were determined. Total cellular RNA and polyadenylated RNA were isolated from SVV-infected and mock-infected Vero cells. Viral transcripts were detected by Northern blot hybridization analysis using overlapping SVV DNA probes representative of the entire SVV genome. The results indicated that all regions of the SVV genome are transcribed during SVV infection in vitro . At least 53 distinct viral RNA species ranging in size from 9·2 to 0·8 kb were detected. DNA probes derived from the SVV DNA long (L) and short (S) components hybridized to 44 RNAs (9·2 to 0·8 kb) and nine RNAs (4·9 to 0·8 kb), respectively. A transcript map of the SVV genome was constructed. The comparison made between the transcript maps of SVV and varicellazoster virus (VZV) provides further support that the SVV and VZV genomes have an analogous gene organization.
Received 3 February 1993;
accepted 10 March 1993.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>8393915</pmid><doi>10.1099/0022-1317-74-8-1639</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Biological and medical sciences Cercopithecus aethiops Fundamental and applied biological sciences. Psychology Gene Expression - genetics Genetics Herpesvirus 3, Human - genetics Microbiology Restriction Mapping RNA, Messenger - genetics RNA, Viral - genetics Transcription, Genetic - genetics Vero Cells Virology |
title | Characterization and mapping of simian varicella virus transcripts |
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