Refined localization and yeast artificial chromosome (YAC) contig—mapping of genes and DNA segments in the 7q21–q32 region

The chromosome localizations for 159 gene and DNA segments have been refined to one of five intervals in the 7q21–q32 region through hybridization analysis with a panel of somatic cell hybrid lines. Seventy-two of these chromosome 7 markers are also mapped on common or overlapping yeast artificial c...

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Bibliographic Details
Published in:Human molecular genetics 1993-06, Vol.2 (6), p.751-760
Main Authors: Scherer, Stephen W., Rommens, Johanna M., Soder, Sylvia, Wong, Ed, Plavsic, Natasa, Tompkins, Brock J.F., Beattie, Aaron, Kim, Julia, Tsui, Lap-Chee
Format: Article
Language:English
Subjects:
Base Sequence
chromosome 7
Chromosome Walking
Chromosomes, Fungal
Chromosomes, Human, Pair 7
DNA
DNA - genetics
DNA Probes
Gene Library
gene mapping
Genetic Markers
Genome, Human
Humans
Hybrid Cells
man
Molecular Sequence Data
yeast artificial chromosomes
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Summary:The chromosome localizations for 159 gene and DNA segments have been refined to one of five intervals in the 7q21–q32 region through hybridization analysis with a panel of somatic cell hybrid lines. Seventy-two of these chromosome 7 markers are also mapped on common or overlapping yeast artificial chromosome (YAC) clones. In addition, the breakpoints of chromosome rearrangment contained in five of the somatic cell hybrid lines have been defined by flanking probes within YAC contigs. To provide a framework for further mapping of the 7q21–q32 region, we have established the physical order of a set of reference markers: cen-(COL1A2-D7S15-CYP3A4-PON)-D7S456-(breakpoint contained in cell hybrid 1EF2/3/K017)-GUSB-D7S186-ASL-(PGY1-PGY3-GNB2-EPO-ACHE)-D7S238-(proximal breakpoint in GM1059-Rag5)-D7S240-(CUTL1-PLANHI)-(breakpoints in 1CF2/5/K016 and 2068Rag22–2)-(PRKAR2B-D7S13)-LAMB1-(breakpoint in JSR-17S)-DLD-D7S16-MET-WNT2-CFTR-D7S8-tel.
ISSN:0964-6906
1460-2083
DOI:10.1093/hmg/2.6.751