Identification of a new hepatitis B virus (HBV) genotype from Brazil that expresses HBV surface antigen subtype adw4

1 Institute of Medical Virology and 2 Department of Virology, Fundaçao Oswaldo Cruz, Rio de Janeiro, Brazil and 3 Department of Pediatrics, Justus-Liebig-University, D-6300 Giessen, Germany The complete genome of a hepatitis B virus (HBV) from Brazil that expressed the subtype adw4 of HBV surface an...

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Bibliographic Details
Published in:Journal of general virology 1993-08, Vol.74 (8), p.1627-1632
Main Authors: Naumann, Heike, Schaefer, Stephan, Yoshida, Clara Fumiko Tachibana, Gaspar, Ana Maria Coimbra, Repp, Reinald, Gerlich, Wolfram H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Brazil
Fundamental and applied biological sciences. Psychology
Gene Expression - genetics
Genetics
Genome, Viral
Genotype
Hepatitis B Surface Antigens - genetics
hepatitis B virus
Hepatitis B virus - classification
Hepatitis B virus - genetics
Humans
Microbiology
Molecular Sequence Data
Open Reading Frames - genetics
Virology
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Summary:1 Institute of Medical Virology and 2 Department of Virology, Fundaçao Oswaldo Cruz, Rio de Janeiro, Brazil and 3 Department of Pediatrics, Justus-Liebig-University, D-6300 Giessen, Germany The complete genome of a hepatitis B virus (HBV) from Brazil that expressed the subtype adw4 of HBV surface antigen (HBsAg) was cloned and sequenced. The genome, termed w4B, consists of 3215 bp. The overall genetic organization of typical hepadnaviruses with four open reading frames including the preC region was found to be conserved. When comparing the w4B sequence with 19 complete HBV genomes it was, however, found to be more divergent (15%) than any other HBV sequence thus far reported. Until now, no more than 11% divergence has been reported. Distinct from the five known HBV genotypes A to E, w4B made up a new, sixth genotype. The importance of the conserved third start codon in the HBV X gene became apparent in isolate w4B. By mutation, this ATG was out of frame, and by what appears to have been a linked mutation, a new start site two codons downstream was re-established. The significance of several other mutations is discussed. Received 11 December 1992; accepted 7 April 1993.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-74-8-1627