Detection of Latent Cytomegalovirus DNA in Diverse Organs of Mice

Latency is essential to the pathogenesis of cytomegalovirus (CMV) infection and disease. A survey of mouse organs that might contain latent murine CMV (MCMV) DNA was conducted using nested enzymatic amplification of a 200-bp region of exon 4 of the major immediate early gene 1. MCMV DNA was detected...

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Bibliographic Details
Published in:The Journal of infectious diseases 1993-09, Vol.168 (3), p.725-729
Main Authors: Collins, Terese, Pomeroy, Claire, Jordan, M. Colin
Format: Article
Language:English
Subjects:
Age Factors
Animals
Antigens, Viral - genetics
Base Sequence
Biological and medical sciences
Concise Communications
Cytomegalovirus
Cytomegalovirus - growth & development
Cytomegalovirus Infections - etiology
DNA, Viral - isolation & purification
Endothelial cells
Experimental viral diseases and models
Female
Immediate-Early Proteins
Infections
Infectious diseases
Kidneys
Liver
Medical sciences
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Nucleic Acid Amplification Techniques
Salivary glands
Spleen
Stromal cells
Tissue Distribution
Viral diseases
Viral DNA
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Description
Summary:Latency is essential to the pathogenesis of cytomegalovirus (CMV) infection and disease. A survey of mouse organs that might contain latent murine CMV (MCMV) DNA was conducted using nested enzymatic amplification of a 200-bp region of exon 4 of the major immediate early gene 1. MCMV DNA was detected in diverse organs including the heart, kidney, liver, lung, spleen, brain, and salivary glands. The total number of organs in which latent MCMV DNA was detected was significantly greater in mice infected at 4 weeks of age (64/70) than in mice infected at 7 weeks of age (40/69). This phenomenon was associated with greater viral replication in the same organs during acute infection. These results indicate that latent MCMV infection is distributed much more widely than previously suspected and is directly correlated with the extent of viral replication during acute infection.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/168.3.725