Association of annexin V with mitochondria

Annexin V is an intracellular protein recently shown to be localized to nucleoli and cytosol. In this study we show that cytosolic annexin V is associated with mitochondria. To assess the nature of the annexin V-mitochondrial interaction, an annexin V binding activity was partially purified from pla...

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Bibliographic Details
Published in:FEBS letters 1993-08, Vol.329 (1), p.79-83
Main Authors: Sun, Jiuru, Bird, Catherina H., Salem, Hatem H., Bird, Phillip
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Annexin A5 - metabolism
Annexin V
Binding and carrier proteins
Biological and medical sciences
Carrier Proteins - isolation & purification
Carrier Proteins - metabolism
Cell Line
Cell structures and functions
Electrophoresis, Polyacrylamide Gel
Female
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Humans
Immunosorbent Techniques
Microscopy, Fluorescence
Mitochondria - metabolism
Mitochondria and cell respiration
Mitochondrion
Molecular and cellular biology
Placenta - chemistry
Proteins
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Summary:Annexin V is an intracellular protein recently shown to be localized to nucleoli and cytosol. In this study we show that cytosolic annexin V is associated with mitochondria. To assess the nature of the annexin V-mitochondrial interaction, an annexin V binding activity was partially purified from placental cytosol by annexin V-affinity chromatography. Five polypeptides in the eluate appeared to be associated with annexin V, with a predominant species of 27 kDa. Antibodies to the 27 kDa polypeptide recognised mitochondria but not nucleoli. We conclude that annexin V interacts with a 27 kDa nutochondrial polypeptide that is possibly part of a larger complex.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80198-4