Multicentre evaluation of a biphasic culture system for recovery of mycobacteria from clinical specimens

A new biphasic system (MB Check, Roche) for isolation of mycobacteria from clinical specimens was evaluated by eight different microbiological laboratories in comparison with methods routinely used in the respective laboratories. Altogether 1125 clinical specimens were processed; pretreatment, if pe...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 1993-06, Vol.12 (6), p.425-429
Main Authors: TORTOLI, E, MANDLER, F, PENATI, V, SIMONETTI, M. T, BARTOLUCCI, M, BERTOLI, G, COSTA, D, GOGLIO, A, INNOCENTI, B, LACCHINI, C, MARCHESANI, F, TOSI, C. P
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriological Techniques
Bacteriology
Biological and medical sciences
Bronchoalveolar Lavage Fluid - microbiology
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Mycobacterium
Mycobacterium - isolation & purification
Mycobacterium Infections - blood
Mycobacterium Infections - microbiology
Mycobacterium Infections - urine
Mycobacterium tuberculosis
Mycobacterium tuberculosis - isolation & purification
Pleural Effusion - microbiology
Prospective Studies
Sensitivity and Specificity
Sputum - microbiology
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Summary:A new biphasic system (MB Check, Roche) for isolation of mycobacteria from clinical specimens was evaluated by eight different microbiological laboratories in comparison with methods routinely used in the respective laboratories. Altogether 1125 clinical specimens were processed; pretreatment, if performed, was by a variety of methods. Mycobacteria were recovered from 167 specimens with the biphasic system and 165 specimens with the other methods. The average time required for isolation of Mycobacterium tuberculosis was 22.6 days with the biphasic system and 24.7 days with egg-based media; for other mycobacterial species it was 23.5 versus 20.8 days. The inclusion of a chocolate agar section in the biphasic system facilitated the early detection of contaminants, while the NAP-containing section appeared unable to differentiate Mycobacterium tuberculosis from other mycobacterial species. The biphasic system, which enables recovery of mycobacteria in small laboratories without specialized equipment, is more practical than conventional methods and at least as sensitive.
ISSN:0934-9723
1435-4373
DOI:10.1007/BF01967436