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The leukocyte surface antigens CD11b and CD18 mediate the oxidative burst activation of human peritoneal macrophages induced by type 1 fimbriated Escherichia coli

Analysis by immunofluorescence‐activated cell sorting of human peritoneal macrophages from patients undergoing intermittent peritoneal dialysis revealed that they express the CD11/CD18 surface antigens, with CDllb and CD18 as the predominant ones. Sodium dodecyl sulfate‐polyacrylamide gel electropho...

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Bibliographic Details
Published in:Journal of leukocyte biology 1993-08, Vol.54 (2), p.111-113
Main Authors: Gbarah, Awni, Gahmberg, Carl G., Boner, Geoffrey, Sharon, Nathan
Format: Article
Language:English
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Summary:Analysis by immunofluorescence‐activated cell sorting of human peritoneal macrophages from patients undergoing intermittent peritoneal dialysis revealed that they express the CD11/CD18 surface antigens, with CDllb and CD18 as the predominant ones. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of im‐ munoprecipitates obtained from lysates of 125I‐labeled macrophages with rabbit polyclonal antibodies against the CDlla‐c/CD18 complex or against CD18, revealed four radioactive bands corresponding to CDlla, CDllb, CDllc, and CD18. Monoclonal antibodies against CDllb and CD18 inhibited by 80 and 90%, respectively, the oxidative burst activation of the macrophages by type 1 fimbriated Escherichia coli, whereas monoclonal antibodies against CDlla, CDllc, and CD43 were without effect. Our results suggest that CDllb and CD18 (receptors for C3bi) serve also as receptors for mannose‐specific E. coli on human peritoneal macrophages and may be involved in the lectinophagocytosis of the bacteria by these cells.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.54.2.111