Quantitative determination of sparfloxacin in rat plasma by liquid chromatography/tandem mass spectrometry

Sparfloxacin, a fluoroquinolone antibiotic, is used for the treatment of bacterial infection. A quantification method using mass spectrometry was developed for the determination of sparfloxacin in rat plasma. After simple protein precipitation with acetonitrile, the analytes were chromatographed on...

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Bibliographic Details
Published in:Biomedical chromatography 2010-11, Vol.24 (11), p.1199-1202
Main Authors: Noh, Keumhan, Kwon, Kwang-il, Jeong, Tae Cheon, Kang, Wonku
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - blood
Anti-Bacterial Agents - chemistry
Chromatography, High Pressure Liquid - methods
Drug Stability
Fluoroquinolones - blood
Fluoroquinolones - chemistry
liquid chromatography
mass spectrometry
rat plasma
Rats
Rats, Sprague-Dawley
sparfloxacin
Tandem Mass Spectrometry - methods
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Summary:Sparfloxacin, a fluoroquinolone antibiotic, is used for the treatment of bacterial infection. A quantification method using mass spectrometry was developed for the determination of sparfloxacin in rat plasma. After simple protein precipitation with acetonitrile, the analytes were chromatographed on a reversed‐phase C18 column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma sparfloxacin concentrations after a single oral administration of sparfloxacin in rats. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.1427