Sialyltransferase activity in FR3T3 cells transformed with ras oncogene: decreased CMP-Neu5Ac:Gal beta 1-3GalNAc alpha-2,3-sialyltransferase

We have investigated the activity of CMP-Neu5Ac:Gal beta 1-3GalNAc alpha-2,3-sialyltransferase (EC 2.4.99.4) in FR3T3 cells transformed by the Ha-ras oncogene in which we have previously demonstrated the higher expression of the beta-galactosidase alpha-2,6-sialyltransferase (EC 2.4.99.1) [21]. We d...

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Bibliographic Details
Published in:Glycoconjugate journal 1993-02, Vol.10 (1), p.91-98
Main Authors: Delannoy, P, Pelczar, H, Vandamme, V, Verbert, A
Format: Article
Language:English
Subjects:
Animals
Arachis - metabolism
Carbohydrate Sequence
Cell Line, Transformed
Cell Transformation, Neoplastic - metabolism
Fibroblasts - enzymology
Gene Expression
Genes, ras
Lectins - metabolism
Molecular Sequence Data
Peanut Agglutinin
Plant Lectins
Rats
Sialyltransferases - metabolism
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Summary:We have investigated the activity of CMP-Neu5Ac:Gal beta 1-3GalNAc alpha-2,3-sialyltransferase (EC 2.4.99.4) in FR3T3 cells transformed by the Ha-ras oncogene in which we have previously demonstrated the higher expression of the beta-galactosidase alpha-2,6-sialyltransferase (EC 2.4.99.1) [21]. We demonstrate that the presence of the activated ras gene decreases the activity of this specific alpha-2,3-sialyltransferase fourfold. According to the kinetic parameters and to mixing experiments, we can assume that this decreased enzymatic activity reflects a decrease in the number of active O-glycan alpha-2,3-sialyltransferase polypeptides in ras-transformed cells. However, no change in the binding of Peanut agglutinin was observed on the cell surface of ras-transformed FR3T3 suggesting that no change in the sialylation of O-glycan core 1 appeared in these cells, although the activity of the alpha-2,3-sialyltransferase was decreased.
ISSN:0282-0080
DOI:10.1007/BF00731192