Separation and Characterization of Dermatan Sulfate in Normal Human Urine

Dermatan sulfate excreted in normal human urine was isolated and characterized by TLC and cellulose acetate strip electrophoresis after cetylpyridinium chloride precipitation and pronase digestion. In these separation methods, dermatan sulfate and chondroitin sulfate were extracted and then monitore...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 1993/04/15, Vol.16(4), pp.340-342
Main Authors: QIU, Guoning, TANIKAWA, Masahiko, AKIYAMA, Hiroshi, TOIDA, Toshihiko, KOSHIISHI, Ichiro, IMANARI, Toshio
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrates
cellulose acetate strip electrophoresis
chondroitin sulfate
Chondroitin Sulfates - isolation & purification
Chondroitin Sulfates - urine
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
dermatan sulfate
Dermatan Sulfate - isolation & purification
Dermatan Sulfate - urine
Electrophoresis, Cellulose Acetate
Fundamental and applied biological sciences. Psychology
glycosaminoglycan
Holosides
human urine
Humans
Indicators and Reagents
Other biological molecules
Spectrometry, Fluorescence
TLC
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Summary:Dermatan sulfate excreted in normal human urine was isolated and characterized by TLC and cellulose acetate strip electrophoresis after cetylpyridinium chloride precipitation and pronase digestion. In these separation methods, dermatan sulfate and chondroitin sulfate were extracted and then monitored by sensitive HPLC methods with post column fluorometric derivatization coupled with chondroitinase ABC, ACII and B digestion. From the results, we demonstrated that human urinary dermatan sulfate contains iduronic acid as its major uronic acid (80-90% of total uronic acid), and is composed mainly of repeated mono-sulfated disaccharide units [Di-4S (structure shown in Fig. 1), 89%] and small numbers of di-sulfated disaccharide units (Di-diSB, 7% and Di-diSE, 1%).
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.16.340