Determination of the detection limit of the polymerase chain reaction for chicken infectious anemia virus

Chicken infectious anemia virus (CIAV) DNA in infected cell cultures and chicken tissues was detected using a polymerase chain reaction (PCR) assay. The complete CIAV genome of several strains was amplified in two segments with two sets of primer pairs. The DNA segments of four CIAV strains and full...

Full description

Saved in:
Bibliographic Details
Published in:Avian diseases 1993-04, Vol.37 (2), p.467-476
Main Authors: Soine, C, Watson, S.K, Rybicki, E, Lucio, B, Nordgren, R.M, Parrish, C.R, Schat, K.A
Format: Article
Language:English
Subjects:
AMPLIFICATION CHAINE POLYMERASE
Anemia - microbiology
Anemia - veterinary
Animals
Base Sequence
Cell culture techniques
Cell lines
Chicken anemia virus
Chickens - microbiology
CULTIVO DE CELULAS
CULTURE DE CELLULE
DNA
DNA probes
DNA Viruses - genetics
DNA Viruses - isolation & purification
DNA, Viral - genetics
DNA, Viral - isolation & purification
Gels
Genome, Viral
Genomes
Molecular Sequence Data
PARVOVIRIDAE
POLLO
Polymerase chain reaction
Polymerase Chain Reaction - veterinary
POULET
Poultry Diseases - microbiology
REACCION DE CADENAS DE POLIMERASA
Sensitivity and Specificity
SISTEMA LINFATICO
SYSTEME LYMPHATIQUE
T lymphocytes
Virus Diseases - microbiology
Virus Diseases - veterinary
Viruses
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chicken infectious anemia virus (CIAV) DNA in infected cell cultures and chicken tissues was detected using a polymerase chain reaction (PCR) assay. The complete CIAV genome of several strains was amplified in two segments with two sets of primer pairs. The DNA segments of four CIAV strains and full-length Cux-1 strain DNA were cloned. After amplification, 100 original genome equivalents were detected by Southern hybridization. The sensitivity of the assay was enhanced considerably by performing a reamplification with nested primers. This modification permitted the detection of one molecule of CIAV DNA. Some problems of the assay and its possible application are discussed
ISSN:0005-2086
1938-4351
DOI:10.2307/1591674