Crystals of a hammerhead ribozyme

Hammerhead ribozyme-inhibitor complexes consisting of an RNA "enzyme" strand and a DNA "inhibitor" strand have been crystallized in four different crystal forms. The crystal form that is most attractive for structure determination diffracts to approximately 3.2 A resolution; it i...

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Bibliographic Details
Published in:The Journal of biological chemistry 1993-09, Vol.268 (26), p.19656-19658
Main Authors: PLEY, H. W, LINDES, D. S, DELUCA-FLAHERTY, C, MCKAY, D. B
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Base Composition
Base Sequence
Biological and medical sciences
Crystallization
DNA - chemical synthesis
DNA - chemistry
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
Molecular Sequence Data
Nucleic Acid Conformation
Oligodeoxyribonucleotides - chemical synthesis
Oligodeoxyribonucleotides - chemistry
RNA, Catalytic - chemical synthesis
RNA, Catalytic - chemistry
RNA, Catalytic - isolation & purification
X-Ray Diffraction - methods
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Summary:Hammerhead ribozyme-inhibitor complexes consisting of an RNA "enzyme" strand and a DNA "inhibitor" strand have been crystallized in four different crystal forms. The crystal form that is most attractive for structure determination diffracts to approximately 3.2 A resolution; it is trigonal, space group P3(1)21 or enantiomorph, a = 92.5 A, c = 185.0 A.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)36566-4