Genetic damage in the bivalve mollusk Corbicula fluminea induced by the water-soluble fraction of gasoline

Although gasoline is an important contaminant of aquatic ecosystems, information concerning the potential effects of this petroleum derivative on the DNA of aquatic biota is lacking. The present study aimed to evaluate the genotoxic and mutagenic effects of acute exposures (6, 24, and 96 h) to gasol...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2010-07, Vol.700 (1), p.80-85
Main Authors: Fedato, R.P., Simonato, J.D., Martinez, C.B.R., Sofia, S.H.
Format: Article
Language:English
Subjects:
Aquatic ecosystems
Asian clam
Bioassays
Biological and medical sciences
Bivalvia
Cells
Comet assay
Corbicula fluminea
DNA damage
Effects
Fundamental and applied biological sciences. Psychology
Gasoline
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Gill cells
Hemocytes
Marine
Medical sciences
Micronuclei test
Mollusks
Nuclear abnormalities
Toxicology
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Summary:Although gasoline is an important contaminant of aquatic ecosystems, information concerning the potential effects of this petroleum derivative on the DNA of aquatic biota is lacking. The present study aimed to evaluate the genotoxic and mutagenic effects of acute exposures (6, 24, and 96 h) to gasoline water-soluble fraction (GWSF), diluted to 5%, on the Asian clam Corbicula fluminea. The comet assay and the micronucleus (MN) test were performed on hemocytes and gill cells of C. fluminea. For the three different times tested, the comet assay indicated DNA damage in hemocytes and gill cells of C. fluminea exposed to GWSF. The MN test detected significant damage in the genetic material of the hemocytes only after 96 h of exposure to GWSF. The recovery capacity of organisms previously exposed for 6 h to GWSF was also evaluated with the comet assay. The results revealed a great capacity of this species to repair DNA damage; following 6 h of recovery, the comet score returned to that of the control groups. Overall, our findings indicate that GWSF has genotoxic and mutagenic effects on C. fluminea. In addition, the present results confirm the sensitivity of C. fluminea to damage caused by exposure to environmental contaminants, and, therefore, its suitability for use in environmental monitoring studies.
ISSN:1383-5718
1879-3592
DOI:10.1016/j.mrgentox.2010.05.012