First molecular detection of a viral pathogen in Ugandan honey bees

Ugandan honey bees ( Apis mellifera L.) produce honey, and are key pollinators within commercial crops and natural ecosystems. Real-time RT-PCR was used to screen immature and adult bees collected from 63 beekeeping sites across Uganda for seven viral pathogens. No samples tested positive for Chroni...

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Bibliographic Details
Published in:Journal of invertebrate pathology 2010-06, Vol.104 (2), p.153-156
Main Authors: Kajobe, Robert, Marris, Gay, Budge, Giles, Laurenson, Lynn, Cordoni, Guido, Jones, Ben, Wilkins, Selwyn, Cuthbertson, Andrew G.S., Brown, Mike A.
Format: Article
Language:English
Subjects:
Animal productions
Animals
Apiculture
Apis
Bees - virology
Biological and medical sciences
BQCV
Crops
Distribution
Fundamental and applied biological sciences. Psychology
Insect Viruses - classification
Insect Viruses - genetics
Insect Viruses - isolation & purification
Insecta
Invertebrates
Microbiology
PCR
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Reverse Transcriptase Polymerase Chain Reaction
Terrestrial animal productions
Uganda
Ugandan honey bees
Viral pathogens
Virology
Virus Diseases - veterinary
Virus Diseases - virology
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Summary:Ugandan honey bees ( Apis mellifera L.) produce honey, and are key pollinators within commercial crops and natural ecosystems. Real-time RT-PCR was used to screen immature and adult bees collected from 63 beekeeping sites across Uganda for seven viral pathogens. No samples tested positive for Chronic bee paralysis virus, Sacbrood virus, Deformed wing virus, Acute bee paralysis virus, Apis iridescent virus or Israeli acute paralysis virus. However, Black queen cell virus (BQCV) was found in 35.6% of samples. It occurred in adults and larvae, and was most prevalent in the Western highlands, accounting for over 40% of positive results nationally.
ISSN:0022-2011
1096-0805
DOI:10.1016/j.jip.2010.02.007