Comparative study of effects of two bile acid derivatives on Staphylococcus aureus by multiple analytical methods

The effects of two bile acid derivatives, cholic acid (CA) and deoxycholic acid (DCA) on Staphylococcus aureus ( S. aureus) growth were investigated and compared by microcalorimetry coupled with multiple analytical methods. The heat power (HP)–time curves of S. aureus growth affected by CA and DCA w...

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Bibliographic Details
Published in:Journal of hazardous materials 2010-07, Vol.179 (1), p.742-747
Main Authors: Kong, Weijun, Jin, Cheng, Xiao, Xiaohe, Zhao, Yanling, Li, Zulun, Zhang, Ping, Liu, Wei, Li, Xing-Feng
Format: Article
Language:English
Subjects:
Analysis of Variance
Applied sciences
Bile acid derivative
Calorimetry
Cholic Acid - pharmacology
Culture Media
Deoxycholic Acid - pharmacology
Derivatives
Exact sciences and technology
Kinetics
MANOVA
Mathematical analysis
Microbial Sensitivity Tests
Microcalorimetry
Nuclear power generation
PCA
Pollution
Principal Component Analysis
Rate constants
S. aureus
Screening
Similarity
Solvents
Staphylococcus aureus
Staphylococcus aureus - drug effects
Steroids
Thermodynamics
Toxicity
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Summary:The effects of two bile acid derivatives, cholic acid (CA) and deoxycholic acid (DCA) on Staphylococcus aureus ( S. aureus) growth were investigated and compared by microcalorimetry coupled with multiple analytical methods. The heat power (HP)–time curves of S. aureus growth affected by CA and DCA were studied by similarity analysis (SA), respectively. Then the quantitative thermo-kinetic parameters obtained from these curves were investigated by the multivariate analysis of variance (MANOVA) and principal component analysis (PCA). By analyzing the two main parameters, growth rate constant k 2 of the second exponential phase and the heat power P 2 of the second highest peak, together with the minimum inhibitory concentration (MIC) values of 10 μg/mL for CA and 20 μg/mL for DCA, it could be concluded that the antibacterial effect of CA was stronger than that of DCA. The existence of α-OH at C-7 position of steroid nucleus of bile acid derivatives enhanced the hydrophilicity of compound CA and its inhibitory effect on S. aureus. This study provides a useful method and idea to accurately evaluate the antibacterial effects of bile acid derivatives, which provides some references for screening out new antibacterial agents with high efficacy and low toxicity.
ISSN:0304-3894
1873-3336
DOI:10.1016/j.jhazmat.2010.03.064