Inhibition of Na currents by the toxin extracts from puffer fishes captured in the sea coast of Japan

The inhibitory effects of toxin extracted from muscle or liver of five different puffer fishes (hereafter referred as puffer(s)) captured on the Japanese sea coast were examined on voltage-dependent sodium current ( I Na) recorded from dissociated single rat hippocampal CA1 neurons. The inhibitory e...

Full description

Saved in:
Bibliographic Details
Published in:Toxicon (Oxford) 2010-11, Vol.56 (6), p.999-1006
Main Authors: Iwata, Satomi, Matsuura, Tatsuya, Yamamoto, Sokatsu, Tahara, Tatsuo, Shin, Min-Chul, Ito, Yushi, Akaike, Norio
Format: Article
Language:English
Subjects:
Animal poisons toxicology. Antivenoms
Animals
Biological and medical sciences
Cells, Cultured
Coastal environments
Edible
Fish
Hippocampal CA1 neuron
Hippocampus - cytology
Inhibition
Inhibitory Concentration 50
Japan
Liver
Liver - chemistry
Marine
Medical sciences
Muscle, Skeletal - chemistry
Muscles
Neurons
Neurons - drug effects
Neurons - metabolism
Patch-Clamp Techniques
Puffer fishes
Rats
Rats, Wistar
Sodium
Sodium Channel Blockers - toxicity
Sodium Channels - drug effects
Sodium Channels - metabolism
Sodium current
Species Specificity
Tetraodontiformes - physiology
Tetrodotoxin
Tetrodotoxin - toxicity
Tissue Extracts - toxicity
Toxicology
Toxin extract
Toxins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The inhibitory effects of toxin extracted from muscle or liver of five different puffer fishes (hereafter referred as puffer(s)) captured on the Japanese sea coast were examined on voltage-dependent sodium current ( I Na) recorded from dissociated single rat hippocampal CA1 neurons. The inhibitory effects estimated from IC 50 values of toxin extracts on I Na were in the order of Takifugu vermicularis >  Lagocephalus wheeleri >  Canthigaster rivulata >  Takifugu rubripes >  Arothron reticularis from muscle and T. vermicularis >  T. rubripes >  L. wheeleri >  A. reticularis >  C. rivulata from liver, thereby indicating that the amount of toxin in the liver or muscle differs between puffers. In addition, the present results indicate that the muscle of T. vermicularis, which is eaten in Japan, contains relatively higher amounts of toxin compared to those of T. rubripes, also eaten. This observation suggests that caution should be taken concerning the maximal edible amount of muscle prepared from T. rubripes.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2010.07.004