An experimental study of use of absorbable plate in combination with self-setting α-tricalcium phosphate for orthognathic surgery

• Published in: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2010-11, Vol.110 (5), p.560-569
• Main Authors: Okabe, Katsuhiko, DDS, Ueki, Koichiro, DDS, PhD, Marukawa, Kohei, DDS, PhD, Mukozawa, Aya, DDS, Miyazaki, Mao, DDS, Nakagawa, Kiyomasa, DDS, PhD
• Format: Article
• Language: English
• Subjects: Absorbable Implants; Animals; Biocompatible Materials - therapeutic use; Biological and medical sciences; Bone Morphogenetic Protein 2 - analysis; Bone Plates; Bone Regeneration - drug effects; Bone Screws; Bone Substitutes - therapeutic use; Calcium Phosphates - therapeutic use; Cell Count; Chondroitin Sulfates - therapeutic use; Coloring Agents; Dentistry; Hydroxyapatites - therapeutic use; Immunohistochemistry; Male; Medical sciences; Models, Animal; Nasal Bone - pathology; Nasal Bone - surgery; Orthognathic Surgical Procedures - instrumentation; Orthognathic Surgical Procedures - methods; Osteoblasts - pathology; Osteoclasts - pathology; Osteogenesis - drug effects; Otorhinolaryngology. Stomatology; Rabbits; Succinates - therapeutic use; Surgery; Time Factors
• ISSN: 1079-2104; 1528-395X
• DOI: 10.1016/j.tripleo.2010.02.033

Objective The purpose of this study was to histologically and immunohistochemically evaluate bone formation using both self-setting α-tricalcium phosphate (α-TCP; Biopex) and absorbable plate (Super Fixsorb-MX) in rabbit cranium bone. Study design Twelve adult male Japanese white rabbits (12-16 wk, 2.5-3.0 kg) were used. The surgical defects were made in the nasal bone of a rabbit, and Biopex was implanted in the left side and no material in the right side. Two-hole absorbable plate and 2 screws (Super Fixsorb-MX) were fixed across the defect in each side. The rabbits were killed at 1, 4, 12, and 24 weeks after surgery, and formalin-fixed specimens were embedded in acrylic resin. The specimens were stained with hematoxylin and eosin. For immunohistochemical analysis, the specimens were treated with bone morphogenetic protein 2 (BMP-2) antibodies. Finally, these were evaluated microscopically. Results New bone formation was observed in the region of absorbable plate and nasal membrane after >4 weeks. The area of new bone with Biopex was significantly larger than that of the control side after 1, 4, and 12 weeks ( P < .05). The number of BMP-2–stained cells in the experimental side was significantly larger than in the control side after 4 and 12 weeks ( P < .05). Conclusion This study suggests that the use of absorbable plate (Super Fixsorb-MX) in combination with Biopex could be useful and that both of Super Fixsorb-MX and Biopex could provide adequate bone regeneration.