Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes

The enhanced metabolism of phosphoinositides, which is associated with a wide variety of stimuli and physiological responses, has been studied intensively. Berridge and his collaborators 1–3 demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrol...

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Bibliographic Details
Published in:Nature (London) 1985-01, Vol.313 (5998), p.141-143
Main Authors: Oron, Y, Dascal, N, Nadler, E, Lupu, M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium - physiology
Cell physiology
Chlorides - physiology
Electric Conductivity
Female
Fundamental and applied biological sciences. Psychology
Humanities and Social Sciences
Inositol 1,4,5-Trisphosphate
Inositol Phosphates - pharmacology
Ion Channels - physiology
letter
Molecular and cellular biology
multidisciplinary
Neurotransmission
Oocytes - physiology
Receptors, Muscarinic - physiology
Science
Science (multidisciplinary)
Sugar Phosphates - pharmacology
Xenopus laevis
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Summary:The enhanced metabolism of phosphoinositides, which is associated with a wide variety of stimuli and physiological responses, has been studied intensively. Berridge and his collaborators 1–3 demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P 2 ), and that the product of this reaction, inositol 1,4,5-trisphosphate (Ins(1,4,5)P 3 ), could cause a release of non-mitochondrial calcium. These findings have been verified in other systems 4–6 . Although the relationship between the hydrolysis of PtdIns(4,5)P 2 and the mobilization of intracellular calcium was clearly demonstrated, the direct link between Ins(1,4,5)P 3 production and the physiological response was only implied. We have investigated the possibility that the intracellular release of Ins(1,4,5)P 3 mediates the muscarinic–cholinergic response is Xenopus oocytes, and we show here that intracellularly injected Ins(1,4,5)P 3 mimics the muscarinic depolarizing chloride current in Xenopus oocytes. This is the first demonstration of a direct link between phosphoinositides metabolism and a neuro-transmitter-induced physiological response.
ISSN:0028-0836
1476-4687
DOI:10.1038/313141a0