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Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes
The enhanced metabolism of phosphoinositides, which is associated with a wide variety of stimuli and physiological responses, has been studied intensively. Berridge and his collaborators 1–3 demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrol...
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| Published in: | Nature (London) 1985-01, Vol.313 (5998), p.141-143 |
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| Main Authors: | , , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c430t-50cc8d70dc3a0e383cf761ad770ea73e47bf1984ca0ea3babe0479b7f248bb3f3 |
| container_end_page | 143 |
| container_issue | 5998 |
| container_start_page | 141 |
| container_title | Nature (London) |
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| creator | Oron, Y Dascal, N Nadler, E Lupu, M |
| description | The enhanced metabolism of phosphoinositides, which is associated with a wide variety of stimuli and physiological responses, has been studied intensively. Berridge and his collaborators
1–3
demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P
2
), and that the product of this reaction, inositol 1,4,5-trisphosphate (Ins(1,4,5)P
3
), could cause a release of non-mitochondrial calcium. These findings have been verified in other systems
4–6
. Although the relationship between the hydrolysis of PtdIns(4,5)P
2
and the mobilization of intracellular calcium was clearly demonstrated, the direct link between Ins(1,4,5)P
3
production and the physiological response was only implied. We have investigated the possibility that the intracellular release of Ins(1,4,5)P
3
mediates the muscarinic–cholinergic response is
Xenopus
oocytes, and we show here that intracellularly injected Ins(1,4,5)P
3
mimics the muscarinic depolarizing chloride current in
Xenopus
oocytes. This is the first demonstration of a direct link between phosphoinositides metabolism and a neuro-transmitter-induced physiological response. |
| doi_str_mv | 10.1038/313141a0 |
| format | article |
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1–3
demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P
2
), and that the product of this reaction, inositol 1,4,5-trisphosphate (Ins(1,4,5)P
3
), could cause a release of non-mitochondrial calcium. These findings have been verified in other systems
4–6
. Although the relationship between the hydrolysis of PtdIns(4,5)P
2
and the mobilization of intracellular calcium was clearly demonstrated, the direct link between Ins(1,4,5)P
3
production and the physiological response was only implied. We have investigated the possibility that the intracellular release of Ins(1,4,5)P
3
mediates the muscarinic–cholinergic response is
Xenopus
oocytes, and we show here that intracellularly injected Ins(1,4,5)P
3
mimics the muscarinic depolarizing chloride current in
Xenopus
oocytes. This is the first demonstration of a direct link between phosphoinositides metabolism and a neuro-transmitter-induced physiological response.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/313141a0</identifier><identifier>PMID: 2578219</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biological and medical sciences ; Calcium - physiology ; Cell physiology ; Chlorides - physiology ; Electric Conductivity ; Female ; Fundamental and applied biological sciences. Psychology ; Humanities and Social Sciences ; Inositol 1,4,5-Trisphosphate ; Inositol Phosphates - pharmacology ; Ion Channels - physiology ; letter ; Molecular and cellular biology ; multidisciplinary ; Neurotransmission ; Oocytes - physiology ; Receptors, Muscarinic - physiology ; Science ; Science (multidisciplinary) ; Sugar Phosphates - pharmacology ; Xenopus laevis</subject><ispartof>Nature (London), 1985-01, Vol.313 (5998), p.141-143</ispartof><rights>Springer Nature Limited 1985</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-50cc8d70dc3a0e383cf761ad770ea73e47bf1984ca0ea3babe0479b7f248bb3f3</citedby><cites>FETCH-LOGICAL-c430t-50cc8d70dc3a0e383cf761ad770ea73e47bf1984ca0ea3babe0479b7f248bb3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9144758$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2578219$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oron, Y</creatorcontrib><creatorcontrib>Dascal, N</creatorcontrib><creatorcontrib>Nadler, E</creatorcontrib><creatorcontrib>Lupu, M</creatorcontrib><title>Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>The enhanced metabolism of phosphoinositides, which is associated with a wide variety of stimuli and physiological responses, has been studied intensively. Berridge and his collaborators
1–3
demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P
2
), and that the product of this reaction, inositol 1,4,5-trisphosphate (Ins(1,4,5)P
3
), could cause a release of non-mitochondrial calcium. These findings have been verified in other systems
4–6
. Although the relationship between the hydrolysis of PtdIns(4,5)P
2
and the mobilization of intracellular calcium was clearly demonstrated, the direct link between Ins(1,4,5)P
3
production and the physiological response was only implied. We have investigated the possibility that the intracellular release of Ins(1,4,5)P
3
mediates the muscarinic–cholinergic response is
Xenopus
oocytes, and we show here that intracellularly injected Ins(1,4,5)P
3
mimics the muscarinic depolarizing chloride current in
Xenopus
oocytes. This is the first demonstration of a direct link between phosphoinositides metabolism and a neuro-transmitter-induced physiological response.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - physiology</subject><subject>Cell physiology</subject><subject>Chlorides - physiology</subject><subject>Electric Conductivity</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humanities and Social Sciences</subject><subject>Inositol 1,4,5-Trisphosphate</subject><subject>Inositol Phosphates - pharmacology</subject><subject>Ion Channels - physiology</subject><subject>letter</subject><subject>Molecular and cellular biology</subject><subject>multidisciplinary</subject><subject>Neurotransmission</subject><subject>Oocytes - physiology</subject><subject>Receptors, Muscarinic - physiology</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Sugar Phosphates - pharmacology</subject><subject>Xenopus laevis</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><recordid>eNptkEFP3DAQRi1URLeAxB9olQOqQNrAOHZi51ihFhYhcQGJW-Q4E2qU2KknOfDvcbXbPfVgzeE9fZYeY2ccrjgIfS244JIbOGArLlWVy0qrT2wFUOgctKg-sy9EbwBQciWP2FFRKl3wesXuNz6Qm8OQ8bVcl_kcHU2_Q3pmxmx0o7OUjQtZE513NotIU_CEmfPZC_owLZSFYN9npBN22JuB8HR3j9nzr59PN3f5w-Pt5ubHQ26lgDkvwVrdKeisMIBCC9uriptOKUCjBErV9rzW0iZqRGtaBKnqVvWF1G0renHMvm93pxj-LEhzMzqyOAzGY1ioUWUtihqqJF5sRRsDUcS-maIbTXxvODR_szX_siX1625zaUfs9uKuU-LnO25SiqGPxltHe63mUqpSJ-1yq1Ei_hVj8xaW6FON_335bet6My8R91t74QMUEIuX</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Oron, Y</creator><creator>Dascal, N</creator><creator>Nadler, E</creator><creator>Lupu, M</creator><general>Nature Publishing Group UK</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes</title><author>Oron, Y ; Dascal, N ; Nadler, E ; Lupu, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-50cc8d70dc3a0e383cf761ad770ea73e47bf1984ca0ea3babe0479b7f248bb3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - physiology</topic><topic>Cell physiology</topic><topic>Chlorides - physiology</topic><topic>Electric Conductivity</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humanities and Social Sciences</topic><topic>Inositol 1,4,5-Trisphosphate</topic><topic>Inositol Phosphates - pharmacology</topic><topic>Ion Channels - physiology</topic><topic>letter</topic><topic>Molecular and cellular biology</topic><topic>multidisciplinary</topic><topic>Neurotransmission</topic><topic>Oocytes - physiology</topic><topic>Receptors, Muscarinic - physiology</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Sugar Phosphates - pharmacology</topic><topic>Xenopus laevis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oron, Y</creatorcontrib><creatorcontrib>Dascal, N</creatorcontrib><creatorcontrib>Nadler, E</creatorcontrib><creatorcontrib>Lupu, M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oron, Y</au><au>Dascal, N</au><au>Nadler, E</au><au>Lupu, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>313</volume><issue>5998</issue><spage>141</spage><epage>143</epage><pages>141-143</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><coden>NATUAS</coden><abstract>The enhanced metabolism of phosphoinositides, which is associated with a wide variety of stimuli and physiological responses, has been studied intensively. Berridge and his collaborators
1–3
demonstrated that the first measurable reaction following cell membrane receptor activation is a rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P
2
), and that the product of this reaction, inositol 1,4,5-trisphosphate (Ins(1,4,5)P
3
), could cause a release of non-mitochondrial calcium. These findings have been verified in other systems
4–6
. Although the relationship between the hydrolysis of PtdIns(4,5)P
2
and the mobilization of intracellular calcium was clearly demonstrated, the direct link between Ins(1,4,5)P
3
production and the physiological response was only implied. We have investigated the possibility that the intracellular release of Ins(1,4,5)P
3
mediates the muscarinic–cholinergic response is
Xenopus
oocytes, and we show here that intracellularly injected Ins(1,4,5)P
3
mimics the muscarinic depolarizing chloride current in
Xenopus
oocytes. This is the first demonstration of a direct link between phosphoinositides metabolism and a neuro-transmitter-induced physiological response.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>2578219</pmid><doi>10.1038/313141a0</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1985-01, Vol.313 (5998), p.141-143 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75932906 |
| source | Nature Journals |
| subjects | Animals Biological and medical sciences Calcium - physiology Cell physiology Chlorides - physiology Electric Conductivity Female Fundamental and applied biological sciences. Psychology Humanities and Social Sciences Inositol 1,4,5-Trisphosphate Inositol Phosphates - pharmacology Ion Channels - physiology letter Molecular and cellular biology multidisciplinary Neurotransmission Oocytes - physiology Receptors, Muscarinic - physiology Science Science (multidisciplinary) Sugar Phosphates - pharmacology Xenopus laevis |
| title | Inositol 1,4,5-trisphosphate mimics muscarinic response in Xenopus oocytes |
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