Application of flow cytometry to the study of antiphagocytic properties of Klebsiella pneumoniae capsular polysaccharide

We used flow cytometry to compare the effects of whole cells and capsular polysaccharides of Klebsiella pneumoniae on the phagocytic ability ot polymorphonuclear leukocytes. Our results showed a light descrease in phagocytic activity in the presence of capsular polysaccharides, but a marked decrease...

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Bibliographic Details
Published in:FEMS immunology and medical microbiology 1993-06, Vol.7 (1), p.63-66
Main Authors: Ruiz, C., Sabio, J.M., Santos, J.L., Montes, M.J., Garcia Olivares, E.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Humans
Klebsiella pneumoniae
Klebsiella pneumoniae - immunology
Microbiology
Neutrophils - immunology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phagocytic activity
Phagocytosis
Polysaccharides, Bacterial - physiology
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Summary:We used flow cytometry to compare the effects of whole cells and capsular polysaccharides of Klebsiella pneumoniae on the phagocytic ability ot polymorphonuclear leukocytes. Our results showed a light descrease in phagocytic activity in the presence of capsular polysaccharides, but a marked decrease with whole cells. Our findings suggest that the resistance to phagocytosis in these microorganisms is not due exclusively to their capsule, as claimed by other authors.
ISSN:0928-8244
1574-695X
DOI:10.1111/j.1574-695X.1993.tb00382.x