Selective neuronal cell attachment to a covalently patterned monoamine on fluorinated ethylene propylene films

The patterned covalent surface addition of a monoamine to fluorinated ethylene propylene films (FEP) controls both cellular attachment and differentiation in defined media conditions. A radio frequency glow discharge (RFGD) process was used to replace FEP surface fluorine atoms with hydroxyl groups....

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Bibliographic Details
Published in:Journal of biomedical materials research 1993-07, Vol.27 (7), p.917-925
Main Authors: Ranieri, John P., Bellamkonda, Ravi, Jacob, Jules, Vargo, Terrence G., Gardella, Joseph A., Aebischer, Patrick
Format: Article
Language:English
Subjects:
Adsorption
Albumins - pharmacokinetics
Albumins - pharmacology
Animals
Biological and medical sciences
Cell Adhesion - drug effects
Cell Differentiation - drug effects
Cell interactions, adhesion
Cells, Cultured
Endothelium, Vascular - cytology
Fibronectins - pharmacokinetics
Fibronectins - pharmacology
Fundamental and applied biological sciences. Psychology
Mice
Microscopy, Electron, Scanning
Molecular and cellular biology
Neuroblastoma
Neurons - cytology
Polyethylenes
Propylamines
Rats
Silanes
Tumor Cells, Cultured
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Summary:The patterned covalent surface addition of a monoamine to fluorinated ethylene propylene films (FEP) controls both cellular attachment and differentiation in defined media conditions. A radio frequency glow discharge (RFGD) process was used to replace FEP surface fluorine atoms with hydroxyl groups. The primary amine was then covalently attached by polymerizing aminopropyl‐triethoxysilane (APTES) via the hydroxyl functionalities. The selective attachment of cells to the APTES regions was determined to be dependent upon the initial adsorption of albumin to the patterned FEP membrane. Albumin was determined to enhance cellular attachment to the APTES regions and prevent attachment to the unmodified FEP areas for both an NB2a neuroblastoma cell line and primary rat endothelial cells. If albumin were not preadsorbed onto the membrane, selective attachment to the modified regions would not occur. Radiolabeling albumin with 125I demonstrated the preference of albumin for adsorption onto the monoamine surface where the cells preferentially attached. Both hydrophobic and ionic forces contributed to the adsorption process. Although selective cellular attachment to the patterned APTES regions could be achieved by albumin preadsorption to the surface, the neuroblastoma cells did not significantly differentiate unless additional serum components were supplemented to the media. © 1993 John Wiley & Sons, Inc.
ISSN:0021-9304
1097-4636
DOI:10.1002/jbm.820270711