Non-lymphoid cells of bronchus-associated lymphoid tissue of the rat in situ and in suspension: with special reference to interdigitating and follicular dendritic cells

Immunohistochemical, enzyme-histochemical and electron-microscopical methods were used to study non-lymphoid cells of control and stimulated rat bronchus associated lymphoid tissue (BALT) in situ and in suspensions. Particular attention was paid to the so-called antigen-handling cells, i.e., the int...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue research 1985, Vol.239 (1), p.177-182
Main Authors: VAN DER BRUGGE-GAMELKOORN, G. J, VAN DE ENDE, M. B, SMINIA, T
Format: Article
Language:English
Subjects:
Air breathing
Animals
Antibodies, Monoclonal
Biological and medical sciences
Bronchi - enzymology
Bronchi - immunology
Bronchi - ultrastructure
Fundamental and applied biological sciences. Psychology
Histocytochemistry
Immunochemistry
Lymphoid Tissue - enzymology
Lymphoid Tissue - immunology
Lymphoid Tissue - ultrastructure
Male
Rats
Rats, Inbred Strains
Respiratory system: anatomy, metabolism, gas exchange, ventilatory mechanics, respiratory hemodynamics
Vertebrates: respiratory system
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Immunohistochemical, enzyme-histochemical and electron-microscopical methods were used to study non-lymphoid cells of control and stimulated rat bronchus associated lymphoid tissue (BALT) in situ and in suspensions. Particular attention was paid to the so-called antigen-handling cells, i.e., the interdigitating cells (IDC), which are situated in the T-cell areas, the follicular dendritic cells (FDC), which appear to be restricted to germinal centers, and macrophages, present both in T-cell and B-cell areas. The interdigitating cells were distinguished by being Ia-positive and by the presence of acid phosphatase and non-specific esterase activity in an area near the nucleus. Follicular dendritic cells could be observed in situ by using a monoclonal antibody and by the in vitro trapping of HRP-anti-HRP complexes. Several types of macrophages were found. At the electron-microscopical level no well-developed IDC and FDC could be detected in control BALT. However, in BALT of lipopolysaccharide-stimulated and mycoplasma-infected rats, well-developed IDC and FDC were found. It can be concluded that IDC's and FDC's can be found in BALT.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00214917