The secondary antibody response induced in tissue cultures

• Published in: Folia microbiologica 1993-01, Vol.38 (3), p.267-272
• Main Authors: STERZL, J, MILEROVA, J, MACKOVA, L, TRAVNICEK, J
• Format: Article
• Language: English
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Antibody Formation; Antibody production; B-Lymphocytes - immunology; Biological and medical sciences; Cells, Cultured; Culture Techniques; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; Immunoglobulin G - biosynthesis; Immunoglobulin M - biosynthesis; Immunologic Memory; Mice; Mice, Inbred BALB C; Rabbits; Spleen - metabolism
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/BF02814390

The conditions for induction of memory cells (B-MC) and evocation of the secondary antibody (Ab) response in tissue cultures (TC) were estimated. (1) In vivo primed B-MC cells were isolated 6-150 d after priming and stimulated in TC with different doses of sheep red blood cell (SRBC) antigen. The Ab response has a strict time and dose dependence: only small doses (10(5)) evoke a secondary response, high doses (10(8), 10(9)) a state of immediate tolerance. (2) Antigen added to TC directly with B-MC rescued their Ab production for a long period. Addition of the antigen 1 or 2 d after setting the TC, follows the Ab-response decay, comparable with virgin cells (B-ICC). (3) Primed B-MC stimulated in TC responded preferentially with an IgM secondary response; the same cell suspension adoptively transferred into isologous recipients switched into IgG cells. (4) Virgin, immunocompetent, B-ICC were primarily stimulated in TC with a small dose of antigen (10(5) SRBC); after 7 d of cultivation the cells were transferred into isologous recipients, SCID mice and into TC. In all cases, the secondary response of IgM was determined, 10 times higher than in the primary controls.