Induction of neovascularization and nonlymphoid mesenchymal cell proliferation by macrophage cell lines

The mature murine macrophage‐like cells NCTC‐3749 and J‐774, the Immature human macrophage‐like cells U‐937‐1, and their conditioned media exhibited potent angiogenic activity in rat corneas and stimulated proliferation of bovine aortic endothelial cells (BAEC) and DNA synthesis in BALB/C‐3T3 cells...

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Bibliographic Details
Published in:Journal of leukocyte biology 1985-03, Vol.37 (3), p.279-288
Main Authors: Polverini, Peter J., Leibovich, S. Joseph
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Capillaries - growth & development
Cell Division
Cell interactions, adhesion
Cell Line
Cornea - blood supply
Culture Media
DNA - biosynthesis
endothelial cell
Fundamental and applied biological sciences. Psychology
growth factor
Macrophages - physiology
macrophge cell lines
Male
Molecular and cellular biology
neovascularization
Rats
Rats, Inbred F344
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Summary:The mature murine macrophage‐like cells NCTC‐3749 and J‐774, the Immature human macrophage‐like cells U‐937‐1, and their conditioned media exhibited potent angiogenic activity in rat corneas and stimulated proliferation of bovine aortic endothelial cells (BAEC) and DNA synthesis in BALB/C‐3T3 cells in culture. In contrast, the immature human macrophage‐like cells HL‐60 and their conditioned media either failed to produce or release detectable quantities of these activities. Exposure of HL‐60 cells to phorbol‐myristate‐acetate (PMA) did not enhance expression of angiogenic and growth stimulating activities by these cells. Both the angiogenic and growth stimulating activities appear to be mediated by a factor(s) that has biochemical properties in common with macrophage‐derived growth factor (MDGF) produced by normal rat peritoneal macrophages. These results suggest that NCTC‐3749, J‐774, and U‐937‐1 macrophage‐like cell lines may be a useful source for the large scale production and characterization of MDGF and macrophage‐derived angiogenic activity.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.37.3.279