Proteasome activity correlates with male BMI and contributes to the differentiation of adipocyte in hADSC

We have previously reported that 26S proteasome subunit mRNA expressions correlate with male body mass index (BMI). In this study, to investigate whether proteasome activities are correlated with BMI, we recruited 61 healthy young Japanese male subjects, measured proteasome activities in their plasm...

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Bibliographic Details
Published in:Endocrine 2010-04, Vol.37 (2), p.274-279
Main Authors: Sakamoto, Kozue, Sato, Youichi, Sei, Masako, Ewis, Ashraf A., Nakahori, Yutaka
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - drug effects
Adipocytes - metabolism
Adipogenesis - drug effects
Adipogenesis - physiology
Adult
Asian Continental Ancestry Group
Body Mass Index
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cell Survival - drug effects
Cell Survival - physiology
Cells, Cultured
Cysteine Proteinase Inhibitors - pharmacology
Diabetes
Endocrinology
Humanities and Social Sciences
Humans
Internal Medicine
Leupeptins - pharmacology
Male
Medicine
Medicine & Public Health
multidisciplinary
Obesity - metabolism
Obesity - physiopathology
Original Article
Proteasome Endopeptidase Complex - blood
Proteasome Inhibitors
Science
Stem Cells - cytology
Stem Cells - drug effects
Stem Cells - metabolism
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Summary:We have previously reported that 26S proteasome subunit mRNA expressions correlate with male body mass index (BMI). In this study, to investigate whether proteasome activities are correlated with BMI, we recruited 61 healthy young Japanese male subjects, measured proteasome activities in their plasma, and correlated them with their BMI and various metabolic factors. We found that among three different proteasome activities, chymotrypsin-like activity in plasma was positively correlated with BMI in healthy Japanese male subjects. Furthermore, we analyzed proteasome activity in vitro during the differentiation of human adipose-derived stem cell (hADSC) into mature adipocytes. In the early stage of differentiation, proteasome activity was at its highest level, and proteasome inhibitor could inhibit hADSC adipocyte differentiation. Our findings suggest that proteasome is an important controlling factor for the development of obesity and adipogenesis.
ISSN:1355-008X
1559-0100
DOI:10.1007/s12020-009-9298-4