Supercritical fluid chromatographic determination of cholesterol and cholesteryl esters in serum on ODS-silica gel column

Cholesterol and cholesteryl esters in human serum were determined by supercritical fluid chromatography on an inert ODS-silica gel column using supercritical carbon dioxide as a mobile phase without a modifier. Chromatograms were obtained by monitoring the eluent simultaneously with an FID and UV de...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1993-08, Vol.65 (15), p.1994-1997
Main Authors: Nomura, Akira, Yamada, Joseph, Takatsu, Akiko, Horimoto, Yoshiyuki, Yarita, Takashi
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chemistry
Cholesterol
Cholesterol - blood
Cholesterol Esters - blood
Chromatography, Liquid
Fluids
Fundamental and applied biological sciences. Psychology
Humans
Spectrophotometry, Ultraviolet
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Summary:Cholesterol and cholesteryl esters in human serum were determined by supercritical fluid chromatography on an inert ODS-silica gel column using supercritical carbon dioxide as a mobile phase without a modifier. Chromatograms were obtained by monitoring the eluent simultaneously with an FID and UV detector at the wavelength of 190 nm. The retention behavior of cholesterol and cholesteryl esters was investigated in terms of the density of CO2 mobile phase. The separation mode was found to be reversed phase, as in liquid chromatography. The amounts of cholesterol and cholesteryl esters extracted from human serum reference material (NIST SRM 909) were determined individually using cholesteryl laurate as an internal standard to give good agreement of total cholesterol with the value certified by NIST.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00063a011