Development of a novel subunit vaccine that protects cotton rats against both human respiratory syncytial virus and human parainfluenza virus type 3

• Published in: Journal of general virology 1993-09, Vol.74 (9), p.1995-1999
• Main Authors: Homa, Fred L, Brideau, Roger J, Lehman, Donna J, Thomsen, Darrell R, Olmsted, Robert A, Wathen, Michael W
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Antibodies, Viral - blood; Baculoviridae - genetics; Base Sequence; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genetic Vectors; HN Protein; Humans; Immunoglobulin G - blood; Lung - microbiology; Macromolecular Substances; Microbiology; Molecular Sequence Data; Neutralization Tests; Parainfluenza Virus 3, Human - immunology; Paramyxoviridae Infections - immunology; Paramyxoviridae Infections - prevention & control; Recombinant Fusion Proteins - immunology; Respiratory Syncytial Viruses - immunology; Respirovirus Infections - immunology; Respirovirus Infections - prevention & control; Restriction Mapping; Sigmodon hispidus; Sigmodontinae; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic; Viral Envelope Proteins; Viral Fusion Proteins - immunology; Viral Proteins - immunology; Viral Vaccines; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-74-9-1995

1 Molecular Biology Research and 2 Cancer and Infectious Disease Research, The Upjohn Company, Kalamazoo, Michigan 49001, U.S.A. A cotton rat model of experimental human respiratory syncytial virus (RSV) and human parainfluenza virus type 3 (PIV-3) infection was used to examine the efficacy of F R HN P , a novel chimeric glycoprotein which contains the extracellular regions of the fusion glycoprotein of RSV and the attachment glycoprotein of PIV-3, as a single subunit vaccine against these two viruses. This work was prompted by previous cotton rat studies that demonstrated that the major protective antigens of the two viruses were these glycoproteins. F R HN P was expressed in insect cells using a recombinant baculovirus. Vaccination with F R HN P resulted in induction of both RSV and PIV-3 neutralizing antibody and doses of 200 ng completely protected rats from either RSV or PIV-3 challenge. These results demonstrate that in the cotton rat animal model a single chimeric glycoprotein can be an effective vaccine against both RSV and PIV-3. Received 26 March 1993; accepted 29 April 1993.