Identification and oligosaccharide structure analysis of rhodopsin glycoforms containing galactose and sialic acid

• Published in: Glycobiology (Oxford) 1993-08, Vol.3 (4), p.365-380
• Main Authors: Duffin, Kevin L, Lange, Gary W., Welply, Joseph K., Florman, Richard, O'Brien, Paul J., Dell, Anne, Reason, Andrew J., Morris, Howard R., Fliesler, Steven J.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino Acids - analysis; Animals; Asparagine - metabolism; Carbohydrate Sequence; Galactose - analysis; Gas Chromatography-Mass Spectrometry; glycoforms; Glycopeptides - chemistry; Glycopeptides - isolation & purification; Glycoproteins - chemistry; Glycoproteins - metabolism; Glycoside Hydrolases - metabolism; Glycosylation; Molecular Sequence Data; N-Acetylneuraminic Acid; oligosaccharide structure; Oligosaccharides - chemistry; Oxidation-Reduction; Protein Processing, Post-Translational; Rana pipiens; rhodopsin; Rhodopsin - chemistry; Rhodopsin - metabolism; Sialic Acids - analysis; Trypsin - metabolism
• ISSN: 0959-6658; 1460-2423
• DOI: 10.1093/glycob/3.4.365

The N-linked oligosaccharides of frog (Rana pipiens) rhodopsin were analysed by sequential exoglycosidase digestion and gel filtration chromatography, following reductive tritiation. In addition, selected tryptic glycopeptides obtained from frog retinal rod outer segment membranes were examined by electrospray mass spectrometry (ES-MS), fast atom bombardment mass spectrometry (FAB-MS), amino acid sequence and composition analysis, and carbohydrate composition analysis. The amino acid sequence data demonstrated that the glycopeptides were derived from rhodopsin and confirmed the presence of twoN-glycosylation sites, at residues Asn2 and Asn15. The predominant glycan (∼60% of total) had the structure GlcNAcβ1–2Manα1–3(Manα1–6) Manβ1–4GlcNAcβ1–4GlcNAc-(Asn), with the remaining structures containing 1–3 additional hexose residues, as reported previously for bovine rhodopsin. Unlike bovine rhodopsin, however, a sizable fraction of the total giycans of frog rhodopsin also contained sialic acid (NeuAc), with the sialylated oligosaccharides being present exclusively at the Asn2 site. FAB-MS analysis of oligosaccharides released from the Asn2 site gave, among other signals, an abundant quasimolecular ion corresponding to a glycan of composition NeuAc1Hex6HexNAc3 (where Hex is hexose and HexNAc is N-acetylhexosamine), consistent with a hybrid structure. The potential biological implications of these results are discussed in the context of rod outer segment membrane renewal.