Kinetics of Extraction of Proteoglycans from Human Cartilage

Fifty‐one uniformly sliced human patellar cartilage specimens were extracted with physiologic buffer containing inhibitors. Galactosamine‐rich proteoglycans were more easily extracted than glucosamine‐rich proteoglycans. Extraction occurred in 2 phases. There was a large proteoglycan extraction duri...

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Bibliographic Details
Published in:Arthritis and rheumatism 1985-03, Vol.28 (3), p.323-330
Main Authors: Pottenger, Lawrence A., Webb, June E., Lyon, Nancy B.
Format: Article
Language:English
Subjects:
Adult
Aging
Biological and medical sciences
Cartilage, Articular - analysis
Diseases of the osteoarticular system
Humans
Male
Medical sciences
Osteoarthritis
Proteoglycans - analysis
Time Factors
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Summary:Fifty‐one uniformly sliced human patellar cartilage specimens were extracted with physiologic buffer containing inhibitors. Galactosamine‐rich proteoglycans were more easily extracted than glucosamine‐rich proteoglycans. Extraction occurred in 2 phases. There was a large proteoglycan extraction during the first 30 minutes, followed by a steady loss proportional to the square root of time. The amount of proteoglycans extracted during phase 1 was approximately half that of phase 2. Extraction of proteoglycans varied with age, structural integrity, and cartilage thickness. Intact osteoarthritic cartilage had normal proteoglycan extraction.
ISSN:0004-3591
1529-0131
DOI:10.1002/art.1780280313