Cathepsin D messenger RNA is downregulated in human lung cancer

Objectives: Lysosomal proteases cathepsins B and D (CB and CD) play a significant part in cancer progression. For many oncological diseases protein expression levels of CB and CD have been investigated and correlations with tumour characteristics revealed. Meanwhile, there is very little information...

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Bibliographic Details
Published in:Biomarkers 2010-11, Vol.15 (7), p.608-613
Main Authors: Shubin, Andrey V., Demidyuk, Ilya V., Kurinov, Alexander M., Demkin, Vladimir V., Vinogradova, Tatyana V., Zinovyeva, Marina V., Sass, Alexander V., Zborovskaya, Irina B., Kostrov, Sergey V.
Format: Article
Language:English
Subjects:
Base Sequence
Biomarkers, Tumor - genetics
Cathepsin B
cathepsin D
Cathepsin D - genetics
DNA Primers
Down-Regulation
gene expression
Humans
lung cancer
Lung Neoplasms - enzymology
real-time PCR
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
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Summary:Objectives: Lysosomal proteases cathepsins B and D (CB and CD) play a significant part in cancer progression. For many oncological diseases protein expression levels of CB and CD have been investigated and correlations with tumour characteristics revealed. Meanwhile, there is very little information concerning mRNA expression level. Methods: In the present work, data about mRNA levels of CB and CD in human lung cancer was obtained using reverse transcription followed by real-time polymerase chain reaction. Results: For the first time CD and CB mRNA in human lung cancer tumours was quantified. It was shown that CB and CD mRNA levels do not correlate with any tumour characteristics. However, in most analysed tumours, expression of CD mRNA was downregulated compared with adjacent normal tissue (p
ISSN:1354-750X
1366-5804
DOI:10.3109/1354750X.2010.504310